FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83271
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21022.524288167549327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12211.4662967899989192No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.5283952396392502No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.41310900553614105No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAA1780.21375989239951482RNA PCR Primer, Index 33 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT1700.20415270622425574RNA PCR Primer, Index 33 (95% over 22bp)
GAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAA1600.19214372350518188No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCT1300.15611677534796028No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC1220.14650958917270118RNA PCR Primer, Index 15 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC1160.13930419954125686No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.12249162373455344No Hit
GGTATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTG890.10687994619975741No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC850.10207635311212787No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.1008754548402205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)