FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329403
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCGACTGCGGGGCTTTCTTAGTAGAAGGGCATAGATGTAAAGTATTTG27680.8403080724826428No Hit
TATAATTTAAGAGCTTTAATGTATAGTATGTCATTCTCAATCAATTGTGCT23700.7194834291126675No Hit
CCCAAAAGACCTCAAAGAACTCATCTTCTTACACTGTGTAGATTGTTGTAA22570.685178944939785No Hit
GTGGGAGGCTTGGGTCAAGCCTTATAATTTAAGAGCTTTAATGTATAGTAT21100.6405527575644423No Hit
CTTATATAACTACATATATATAAAACTAAAAACACCAGTGCAGCTTCACAT21030.6384277010227594No Hit
CCTTAAGATCAGCATCCTCATTCTCAAGAGCCGTATACACTTCGTAATACT20980.6369098034929858No Hit
CCAATGATCACATTCTAATGCATCCACACGACTTCAACTTGCTTTGCTGCC20740.6296238953500727No Hit
GCAGTCGAAGCACCTGTTGTAGCGCTTTGCCCGTCGGCGCTTAGCACACTT20480.6217308281952502No Hit
GTAAACCCGGTGGGAGGCTTGGGTCAAGCCTTATAATTTAAGAGCTTTAAT17610.5346035099862478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.4453511352355625No Hit
CCATATAATGCTCCAACTGCAGCTTTCATGTTCAAGGAATTGATAATCTCC14650.4447439762236531No Hit
CTTTTGGGAGGGGAGTTTGTGAAAAGTGCAGGCAGCAAAGCAAGTTGAAGT14620.443833237705789No Hit
AGTATAACCTCAGCATTGATATCGGAAACGTCATATACATATAATGAGGTA14580.4426189196819701No Hit
GAGTACATGGGGAGTTTATTATCAAAAACACATGTCAGCTCCAATTGAAAC14300.4341186935152382No Hit
ACCACCAAGTAATGTTTCAATTGGAGCAGCTGTGAATGAACGGGTTTTATT13820.41954687722941203No Hit
CCATTCCAAAAGACCTCAGAACTCTTCTTTTCGAAATCTGTGTTAGTGAAT13390.4064929584733594No Hit
GGTGGGAGGCTTGGGTCAAGCCTTATAATTTAAGAGCTTTAATGTATAGTA13320.4043679019316764No Hit
GTTATATAAGAACGTATGTAATATGTATTCCTCTCCATTTCGTTTAACTTG10920.33150882050254554No Hit
GTTGCAAGGTAAGTTTAGCAATATCAACAGTCGTGAGTGTCTTTGTTCACG10870.329990922972772No Hit
GTGGGAGGCTTGGGTCAAGCCTTGTAGTTCGGAAATAATTTAATGTACAGT10850.3293837639608625No Hit
GAGGTTATACTAGGGTTAAACTCAACACTCACTAGACAAACCTTTTCTCTC9310.2826325200438369No Hit
GCATATGTGAAGGATTTCACAAAGTATTCTAGCGTAATTGAAACTGGCTCT8510.25834615956745993No Hit
ACCAAGATGACGCAAAATTTGCCGGATTTGACTTCTTTAATGCGGTACTTA8120.24650655883522615No Hit
CCTAATTTATCAGGAAACTGGACCATGATGGATGACGAAGAACAAGTTGAG7590.2304168450196264No Hit
ATAATTTAAGAGCTTTAATGTATAGTATGTCATTCTCAATCAATTGTGCTG7480.22707747045412457No Hit
GATCTTAAGGAGGTTCGTTATCAAGCTGGAGAAGGAGAAGATGCAGCAGCA7450.2261667319362604No Hit
TAATTCTTGGAACTGCAAATTTACCGGTTGTGCCAGTGTCAACATCTTTAT7350.22313093687671334No Hit
GATCATTGGAGGCTTGGGCTGTTCATGTATCTGTTGTTGTGCAAGGAGGTC7290.22130945984098505No Hit
GTCTTTTGGGAGGGGAGTTTGTGAAAAGTGCAGGCAGCAAAGCAAGTTGAA7250.22009514181716622No Hit
ACCGACGCCGAGATTCTTGCCCACTCCATGAATGCTAAGATGTCAATTGTG7010.21280923367425314No Hit
CGCAGTCGAAGCACCTGTTGTAGCGCTTTGCCCGTCGGCGCTTAGCACACT6930.2103805976266154No Hit
CCTCTCTTGAGTATATCAATAACATCGTTAATAGAAGCTTCGAACGCATCA6910.20977343861470601No Hit
GATAAATTAGGTGATGTTCCATTCTCAATACACCAAACCATCAAACCATTT6870.20855912059088716No Hit
GCTTGTTGGGAGCTCTTTGTACTCTGGAATACAGAATGTCTACTAAGTATT6800.20643406404920417No Hit
CCTAGTTCGTCAAGGCCTCCCACAAAACTTGAAGGAATCGAGCAAAGCTTA6690.20309468948370235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.2027911099777476No Hit
CCTTTGTTGGGGCATTACGGAAAGAGTATGCTCAACAAACAGGCATATGTG6600.20036247393010995No Hit
CATCTTGGTAGCCTTTCCTTGACCAACTAGCTGGTGGTTTCTTCTGTTGTT6560.1991481559062911No Hit
GCTTAGAGATGGGATTTATATACCGAAGCTAGATAAAGAACGTGTAGTTTC6530.19823741738842693No Hit
TCTACTAAGAAAGCCCCGCAGTCGAAGCACCTGTTGTAGCGCTTTGCCCGT6520.1979338378824722No Hit
GCAGCGATTAGAGGGGCAACTAATCGTTTGTTTGGCCTGGATGGTAACGTA6150.1867013961621479No Hit
GCACCACCTGGAGGAATGAGATTTAAGCCCACCGACGCCGAGATTCTTGCC6120.18579065764428376No Hit
TCAAAATATTCTTTCTTTTTACCTCCATATAATGCTCCAACTGCAGCTTTC6070.1842727601145102No Hit
GCAACGTGTGCTGGTTCTTATTAACATCCGCAGCCGTGTGTCTTTCCGTGT6060.18396918060855547No Hit
CCTCACGCTTCGTTACTCTAAGTACAAAAGCACCTTGTGGAATGCCTCACT5880.17850474950137066No Hit
GTTGCAAGGTAAGTTTAGCAATATCAACAATCGTGGGTGTCTTTGTCCACG5870.17820116999541596No Hit
TCTTAGTAGAAGGGCATAGATGTAAAGTATTTGTGGCAAAAGCTCACTCAG5620.17061168234654814No Hit
CCATACATAGCAGAAACAGCTCTCAAGAAGTTGTTTACTAACGTCGACGCG5610.17030810284059344No Hit
GCTGTGTCGGTAGGTAGTAATGTGGATGGATTCGGCGTAACACTCGGTCCA5580.16939736432272928No Hit
CATATTTGACCAGGTCTTTCATGTAAAATGGCGATAATTCAAAGGCTGCCA5560.1687902053108199No Hit
GCTATGTATGGTGCCTTTCCACTTTGAGCTATCATGTTATACGGAGCTTGC5530.16787946679295573No Hit
TATAGCTTAGCGGGTCCCTCGTGTATGACAGCTAACACATCTGAGTGAGCT5460.16575441025127277No Hit
GCTAAGCTATATGCTGAAAGGTCCTATAGGCCAAATTCTGACGCAGCACAA5430.16484367173340864No Hit
GGTCAAATATGATGGTTCAATGCATAAACACATACATAGTGCTCTGAAGAC5370.16302219469768034No Hit
GCTAATGATGTCGAGTCAAATTCAATAGCCTCGAGAGCGACTATAAGAGAT5330.1618078766738615No Hit
AATACCAAGCAACGTGTGCTGGTTCTTATTAACATCCGCAGCCGTGTGTCT5160.1566470250726314No Hit
CCAATGATCACGTTCTGATGATTCCTCAAGACTTTAATCTACTCTGCTGTT5070.153914809519039No Hit
CACATATGCCTGTTTGTTGAGCATACTCTTTCCGTAATGCCCCAACAAAGG5030.15270049149522014No Hit
GCTAGTTGGTCAAGGAAAGGCTACCAAGATGACGCAAAATTTGCCGGATTT4710.14298594730466935No Hit
GGTTATACTAGGGTTAAACTCAACACTCACTAGACAAACCTTTTCTCTCTC4630.14055731125703166No Hit
GTAAACCCTGTGGGAGGCTTGGGTCAAGCCTTGTAGTTCGGAAATAATTTA4540.13782509570343926No Hit
TCTTTCCTAGGTATAATCGTTTACAACTAGCTTCCAGGATCTTATCATAAT4490.1363071981736657No Hit
CTTAGTTTGGCACGTTACGCATTCGATTTTTATGAGATGACTTCTAAAACT4450.13509288014984686No Hit
ACTTGGTGGTAAGGTTTGTGTTGATGATTTCAATAACAAATTCTATAGCTG4360.13236066459625442No Hit
GCTACGTTGCGGGCAAACAACAAAACAAGCCGCCTGCTAACTGGTCTAGGA4330.1314499260783903No Hit
TGTAAACCCGGTGGGAGGCTTGGGTCAAGCCTTATAATTTAAGAGCTTTAA4290.13023560805457146No Hit
ATCTTTCCTAGGTATAATCGTTTACAACTAGCTTCCAGGATCTTATCATAA4260.12932486953670733No Hit
CGTGTATGACAGCTAACACATCTGAGTGAGCTTTTGCCACAAATACTTTAC4260.12932486953670733No Hit
GAACATCGATTGGAGGCTATATGTGCATCTATGATTGAAGCATGGGGTTAT4220.12811055151288847No Hit
ATGCTATGTTATCCGTATTATGCTTCCATCCTTTCACCCATTCTATAACGT4200.12750339250097903No Hit
GCATTATATGGAGGTAAAAAGAAAGAATATTTTGAAGGGTTTCAGGAAAGC4190.12719981299502434No Hit
CCAGGAGGACTGGGGGAAGAATCGCTAAGCACAGCGTTAAAGAAGTCGAAA4190.12719981299502434No Hit
CCTCCCAAAAGACCTCAAAGAACTCATCTTCTTACACTGTGTAGATTGTTG4070.12355685892356778No Hit
CGTGATACATCACACTCATGAAGCTCAACATTAATCACAGATATTTAACTA4020.12203896139379422No Hit
CGATTTGATCTGGTTTGTAAGCTAACAAGTGTTCTGCATTAAGCACAACGG3970.12052106386402067No Hit
GATCATTGGTGGTTTTGGCTGCTCATGTATTTGTTGCTGCGCAAGTAAATC3870.11748526880447355No Hit
GGCCTATAGGACCTTTCAGCATATAGCTTAGCGGGTCCCTCGTGTATGACA3790.11505663275683585No Hit
CCTGGAGGAATGAGATTTAAGCCCACCGACGCCGAGATTCTTGCCCACTCC3690.11202083769728872No Hit
GCTTACTTATTAGATAATCATGAGTGTAGAATCTTTACATCGCGTGCTCAA3690.11202083769728872No Hit
TCTCTAAGCAGTCCTCTATGTGACATAAAATACACTTCCTCCTTAGATTTG3640.11050294016751516No Hit
CCCATTTGATTGTACTCACGCACTGTGAACCAATCTTTAATACTAGCGTAT3590.10898504263774161No Hit
GAGTACATGGGGCGTTATCATCTCTGACTAAGACAGTTGACAGCATATACT3570.10837788362583217No Hit
GCTCTATATAGTGTTTCCAAATTCTCCTCACTGTGATCAATCATGTACTTC3520.10685998609605862No Hit
GTCTGAAGGTTGGTTTTGCGTTGTCGAGGACTGGTGCTAGTGGATACTCAA3440.10443135004842093No Hit
GCTATATGTGCATCTATGATTGAAGCATGGGGTTATCCCGATCTGTTACAC3440.10443135004842093No Hit
GACCAACTAGCTGGTGGTTTCTTCTGTTGTTTGCCCGTGACGTAACAAGCT3410.10352061153055679No Hit
CCAGAATAAACCTTTCCAAGGTGTTTCTAAGTGTGCTAAGCGCCGACGGGC3390.10291345251864736No Hit
TAACATAGCATGGGGTTCTTTAAAAATTCAAGAGGACGCTCCAGAAGCTTT3390.10291345251864736No Hit
CTTTTGGAATGGAGGGTTTAGCAAAGTGTAAACAGCAGAGTAGATTAAAGT3380.10260987301269266No Hit
AGTATTTACCTCATTATATGTATATGACGTTTCCGATATCAATGCTGAGGT3340.1013955549888738No Hit
GTTACGTCACGGGCAAACAACAGAAGAAACCACCAGCTAGTTGGTCAAGGA3330.1010919754829191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)