FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83441
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25213.021296484941456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13381.6035282415119665No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA9651.1565057945134884RNA PCR Primer, Index 3 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC5980.7166740571182033TruSeq Adapter, Index 3 (96% over 25bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.596828897064992No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT4210.5045481238240194RNA PCR Primer, Index 3 (95% over 21bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.48657134981603767No Hit
CCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAA3050.3655277381622943RNA PCR Primer, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC2530.30320825493462444No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC1830.21931664289737662TruSeq Adapter, Index 3 (95% over 22bp)
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTC1420.17018012727555998No Hit
GAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAA1300.15579870806917462No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.1534018048681104No Hit
ATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC930.11145599884948647No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.11145599884948647No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.11025754724895435No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCT910.10905909564842223No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT880.1054637408468259Illumina PCR Primer Index 12 (96% over 25bp)
GACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAA840.10066993444469745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)