FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1652733
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATATTCCGGTACCTCGATGTGGATTATCCGCGGCAACGCAACTGAAG190091.150155530264114No Hit
GAATATTAACCGGTTTCCCTTTCGCCAGGCGGCGCCCGAGAGCGCACTTTA162510.9832804209754388No Hit
CGTTACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAAC144830.8763060942088045No Hit
ACTAGTAACGATACATTCAAATGTCCACGTTCAACTAAGTAACAAGGACTT139410.8435119284240103No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAG104420.6318019910052016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99000.5990078252204076No Hit
TCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTTTT98170.5939858404231052No Hit
ACTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGA95490.5777702750535023No Hit
GCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTTGACGAT85640.5181720217361183No Hit
GCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGT76620.46359575321603674No Hit
GATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGA75570.4572426399182445No Hit
GTTTCCCTATGGCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACAC74900.4531887485758438No Hit
CGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGA70170.4245694858153131No Hit
TAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGG66430.40194030130698666No Hit
CCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAAAG63360.3833650081410609No Hit
TCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAACTAGAGGCTGT61650.3730185093417993No Hit
CCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTACGT60450.3657578084300368No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57640.34875566712832623No Hit
ACGCGTAATGAAAGTGAACGGAGGTGAGAACCGCAAGGTGCATCATCGACC56450.3415554720574951No Hit
ACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAA54950.3324795959177919No Hit
GCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTA54000.32673154102931323No Hit
AGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGA48170.2914566357663337No Hit
CCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTT46970.28419593485457123No Hit
GTCTACTCGCCGCGTTGCAGGCCAGCATCGTCTGGTGCCGCTGGATAAGAC46580.28183620705824836No Hit
CAGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAG46370.28056558439868995No Hit
CTAATCATTCGCTTTACCTCATAAAACTGAAAACGTTACTGCTATCCTGAG45870.27754029235212224No Hit
GGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCCGGTGCGCTCTC44970.2720947666683003No Hit
GTTCTACCCGGGGTCACCCCCAAGGCTTCGTCACGGGCCTCCACGCCTGCC44500.2692509921445267No Hit
GCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATT43540.2634424314151167No Hit
GCCTACTCGCTAGGGCATCGTTTCTACCCTAGCGGCGAGGTATTGGTAGTA43240.261627256187176No Hit
CTTGTGATGCAGCGAGCGCCGGGCGAGGTCCGCGCTTCGGCTAGGATGCTG42490.2570893181173245No Hit
GCTTCTGAGTGGCCACCGACCTAAGTTCCTTGGAACAGGACGTCATAGAGG42370.25636324802614824No Hit
AATGATTAGAGGCCTTGGGGTTGAAACAACCTTAACCTATTCTCAAACTTT42360.25630274218521687No Hit
GCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACC42080.2546085786391389No Hit
CCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCC41380.25037316977394414No Hit
CCTAGCGGCGAGGTATTGGTAGTACGCTTGAGCGCCATCCATTTTCAGGGC40390.24438309152174006No Hit
AGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGA40270.24365702143056378No Hit
CCGTGTGAACAGCAGTTGGACACGGGTTAGTCGATCCTAAGCCATAGGGAA39980.2419023520435545No Hit
TGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGCCGCTAGGGTAG39960.2417813403616918No Hit
GCATCACAAGAGGCTATAACACTCCCGAGGGAGCTACATTCCTCAAGTCTT39840.24105527027051557No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGC39840.24105527027051557No Hit
CATTACGCGTAGGGGTTTGACACCCGAACACTCGCATAGATGTTAGACTCC38850.2350651920183115No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTC38780.234641651131792No Hit
AGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCTTGGGGGTGACCCCG38630.23373406351782172No Hit
GTTCATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCC37740.22834904367493117No Hit
GCCGCTAGGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCGTGGAGGCCCG37200.22508172826463801No Hit
GGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAA36860.22302452967297198No Hit
GCCGGAATGTACGCTTATCAGACACCACAAAAGGTGTTAGTTCATCTAGAC36460.2206042960357178No Hit
AACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATT35960.21757900398915012No Hit
GCCCTAGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCTTGGGGGTGA35700.21600585212493487No Hit
GCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATA35600.21540079371562132No Hit
TCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCTTA35530.21497725282910185No Hit
AATATTAACCGGTTTCCCTTTCGCCAGGCGGCGCCCGAGAGCGCACTTTAA35000.2117704432597401No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTG34710.21001577387273082No Hit
CCCTAGCGGCGAGGTATTGGTAGTACGCTTGAGCGCCATCCATTTTCAGGG34600.2093502096224859No Hit
GTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATC32380.19591791293572525No Hit
GCCGGAAGGAATGATTTTCACGCGAGGTCGTACTCATAACCGCAGCAGGTC32320.19555487789013715No Hit
GGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAAT32290.19537336036734307No Hit
GCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCTTGGGGGTGACCCCGG32220.19494981948082357No Hit
ACTCTGGTGGAGGCTCGCAGCGGTTCTGACGTGCAAATCGATCGTCAAATT31860.19277160920729483No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTC31290.18932277627420763No Hit
CTCTATACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTA31100.1881731652965119No Hit
CTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGAT31000.18756810688719835No Hit
TCTAATCATTCGCTTTACCTCATAAAACTGAAAACGTTACTGCTATCCTGA30430.18411927395411118No Hit
GCGGATTACGACCATTACGCCAGCATCCTAGCCGAAGCGCGGACCTCGCCC30210.18278814545362138No Hit
TACGCGTAATGAAAGTGAACGGAGGTGAGAACCGCAAGGTGCATCATCGAC30210.18278814545362138No Hit
CCATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGT29500.1784922307474952No Hit
GTTAAAAGGGAAGGGATTGCAACCAGACTTGCTCGCGGTGTTCCGCCGGTC29340.17752413729259356No Hit
TCCCTTTTAACAATTTCACGTGCTTTTTAACTCTCTTTCCAAAGTGCTTTT28630.1732282225864674No Hit
CCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCT28530.17262316417715384No Hit
GTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTA28420.17195759992690896No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTG27540.16663308592494977No Hit
CTTTGGAAAGAGAGTTAAAAAGCACGTGAAATTGTTAAAAGGGAAGGGATT27370.16560448662911675No Hit
CCCTTTTAACAATTTCACGTGCTTTTTAACTCTCTTTCCAAAGTGCTTTTC27010.16342627635558798No Hit
CTTTCCAAAGTGCTTTTCATCTTTCGATCACTCTACTTGTGCGCTATCGGT26180.1584042915582856No Hit
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGC26170.15834378571735422No Hit
ATGGCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTT25830.1562865871256882No Hit
GCTCTATACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCT25710.15556051703451193No Hit
GCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTG25430.153866353488434No Hit
CCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCTTAG24880.1505385322372095No Hit
GGGGGTGACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAG24750.1497519563051019No Hit
GGGAGCAGGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCTGGTGG24560.14860234532740618No Hit
TCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATC24070.14563755912176982No Hit
CGCCGCTAGGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCGTGGAGGCCC23960.14497199487152493No Hit
CCAAGGAGTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGT23810.14406440725755462No Hit
GCGGAACACCGCGAGCAAGTCTGGTTGCAATCCCTTCCCTTTTAACAATTT23690.14333833716637837No Hit
CACTAGTAACGATACATTCAAATGTCCACGTTCAACTAAGTAACAAGGACT23660.1431568196435843No Hit
CCGAAGACATCAGGATCGGTCGATGATGCACCTTGCGGTTCTCACCTCCGT23550.1424912553933394No Hit
CCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTG23490.14212822034775127No Hit
CCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCG22900.13855837573280136No Hit
GCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAAT22450.1358356128908904No Hit
GAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGCCGCTAGGGTAGA22330.13510954279971418No Hit
CCGGAAGGAATGATTTTCACGCGAGGTCGTACTCATAACCGCAGCAGGTCT22130.1338994259810871No Hit
CCTATACCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCGAGCCT22050.13341537925363625No Hit
TCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGA21920.13262880332152865No Hit
GCGAGTAGACCGGTCAGAAGACCGGCGGAACACCGCGAGCAAGTCTGGTTG21710.13135818066197022No Hit
GATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT21690.13123716898010748No Hit
GCTAGGGCATCGTTTCTACCCTAGCGGCGAGGTATTGGTAGTACGCTTGAG21660.13105565145731343No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTT21420.12960351127496092No Hit
GTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTA21280.12875642950192198No Hit
AAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC21240.12851440613819654No Hit
AGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGG21230.1284539002972652No Hit
GCACTAGAGGCCGTTCTACCCGGGGTCACCCCCAAGGCTTCGTCACGGGCC21050.12736479516050084No Hit
GTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAAT21000.12706226595584405No Hit
TCATTACGCGTAGGGGTTTGACACCCGAACACTCGCATAGATGTTAGACTC20970.12688074843304997No Hit
CGTATAGAGCGCCTTTCCGACCGCATACGGGATTCTCACCCTCTATGACGT20890.12639670170559916No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAA20750.1255496199325602No Hit
GTAATCCGCCCGTCTTGAAACACGGACCAAGGAGTCTAACATCTATGCGAG20650.12494456152324664No Hit
GTTAAGGTGCCGGAATGTACGCTTATCAGACACCACAAAAGGTGTTAGTTC20580.12452102063672717No Hit
GCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAATAGGGTGAAGCCAG20150.12191926947667893No Hit
GCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGT20130.12179825779481622No Hit
ATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGA20040.12125370522643403No Hit
GCACCTTAACCTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCA19770.11962004752128746No Hit
GCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGT19750.11949903583942476No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTT19460.1177443664524155No Hit
GGTAGAGCAGCACACCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGA19430.11756284892962142No Hit
CCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTC19420.11750234308869006No Hit
CTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCCGCGG19330.11695779052030787No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19140.11580817954261215No Hit
TTAATATTCCGGTACCTCGATGTGGATTATCCGCGGCAACGCAACTGAAGG19110.11562666201981808No Hit
GTCAGAAGACCGGCGGAACACCGCGAGCAAGTCTGGTTGCAATCCCTTCCC19050.11526362697422995No Hit
ATGATTAGAGGCCTTGGGGTTGAAACAACCTTAACCTATTCTCAAACTTTA19010.11502160361050454No Hit
CCACTAGTAACGATACATTCAAATGTCCACGTTCAACTAAGTAACAAGGAC18900.11435603936025963No Hit
GCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTA18480.11181479404114276No Hit
GATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCC18440.11157277067741735No Hit
TCTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGCAT18320.11084670058624109No Hit
GTTACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACC18200.11012063049506485No Hit
CCAATACCTCGCCGCTAGGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCG18140.10975759544947672No Hit
CATTCCGGCACCTTAACCTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTG18050.10921304288109453No Hit
GGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAG17990.1088500078355064No Hit
GCTCCCTCGGGAGTGTTATAGCCTCTTGTGATGCAGCGAGCGCCGGGCGAG17980.10878950199457506No Hit
GCTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGT17730.10727685597129119No Hit
GGGAAGAGTTCTCTTTTCTTCTTAACTGTCTATCACCCTGAAATCGGTTTG17670.10691382092570306No Hit
GCTTTTTAACTCTCTTTCCAAAGTGCTTTTCATCTTTCGATCACTCTACTT17450.10558269242521327No Hit
CCGGGAAGAGTTCTCTTTTCTTCTTAACTGTCTATCACCCTGAAATCGGTT17320.10479611649310566No Hit
CGTCAAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTG17150.10376751719727265No Hit
GCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGGTT17140.10370701135634128No Hit
GCCGACGTCTCCACCTTCAGTTGCGTTGCCGCGGATAATCCACATCGAGGT17110.10352549383354723No Hit
TCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAG16770.10146829524188118No Hit
ACGTATAGAGCGCCTTTCCGACCGCATACGGGATTCTCACCCTCTATGACG16710.10110526019629305No Hit
CGTCATAGAGGGTGAGAATCCCGTATGCGGTCGGAAAGGCGCTCTATACGT16550.10013716674139139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)