FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253156
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39501.5603027382325523No Hit
GATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGATACTGC39361.5547725513122344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.7390699805653431No Hit
GGATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGATACTG6370.2516235048744648No Hit
GTGCGTGAAGAAGGGAAACAGCTACGGTATCGATATTGTTGAGACCGAGAA6050.23898307762802384No Hit
GCTTTCTTCGAGCCTTTTCGCCTTCCTTCCTCGTCTAACCATGTCGACCTG5720.22594763703013163No Hit
GCTTTCTTCGAGCCTCTTCGCCTTCCTTCCTCGTCTAACCATGTCGACCTG4820.1903964353995165No Hit
GCGGCAACTGCGACTGCGTTGACAAGAGCCAGTGCGTGAAGAAGGGAAACA4660.18407622177629604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4460.17617595474727046No Hit
ACGTCGACGAGGTGATCGTTGCCGCAGAAGCTGCCGAGCATGACGGCAAGT3900.15405520706599882No Hit
CCTCGTCTAACCATGTCGACCTGCGGCAACTGCGACTGCGTTGACAAGAGC3870.152870167011645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3560.14062475311665534No Hit
GCTTCTTCGAGCCTTTTCGCCTTCCTTCCTCGTCTAACCATGTCGACCTGC3350.13232947273617848No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.12679928581586059No Hit
AGAAGAGCTACGTCGACGAGGTGATCGTTGCCGCAGAAGCTGCCGAGCATG2990.11810899208393244No Hit
GGTAGGTAGGTAGGAGATGATTCATCAACCTTACAAGTTAGTTTCTAGAAG2790.11020872505490685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA2690.10625859154039406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)