FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228326
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGATACTGC47052.060650123069646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28981.26923784413514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.5470248679519635No Hit
GTGCGTGAAGAAGGGAAACAGCTACGGTATCGATATTGTTGAGACCGAGAA8070.35344200835647277No Hit
GGATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGATACTG7610.33329537590988323No Hit
GCTTTCTTCGAGCCTTTTCGCCTTCCTTCCTCGTCTAACCATGTCGACCTG7250.31752844616907405No Hit
GCTTTCTTCGAGCCTCTTCGCCTTCCTTCCTCGTCTAACCATGTCGACCTG7100.31095889211040356No Hit
GCGGCAACTGCGACTGCGTTGACAAGAGCCAGTGCGTGAAGAAGGGAAACA5180.2268686001594212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4410.19314488932491264No Hit
ACGTCGACGAGGTGATCGTTGCCGCAGAAGCTGCCGAGCATGACGGCAAGT3850.16861855417254276No Hit
CCTCGTCTAACCATGTCGACCTGCGGCAACTGCGACTGCGTTGACAAGAGC3590.1572313271375139No Hit
GGTAGGTAGGTAGGAGATGATTCATCAACCTTACAAGTTAGTTTCTAGAAG2910.12744934873820765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA2800.12263167576184927No Hit
CCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTCGACAGCGCCTCGT2760.12087979467953715No Hit
GCTTCTTCGAGCCTTTTCGCCTTCCTTCCTCGTCTAACCATGTCGACCTGC2690.11781400278549092No Hit
GGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA2630.11518618116202273No Hit
GCAGTCGGTGCAGGCGCAGGCGGCGCCGCACTTGCACTTGCCGTCATGCTC2590.1134343000797106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2470.1081786568327742No Hit
AGAAGAGCTACGTCGACGAGGTGATCGTTGCCGCAGAAGCTGCCGAGCATG2410.105550835209306No Hit
GAGTGAATGGGGCGGTTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.10160910277410369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)