FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184146
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGATACTGC61243.3256220607561393No Hit
GCTTTCTTCGAGCCTTTTCGCCTTCCTTCCTCGTCTAACCATGTCGACCTG13460.730941752739674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.6592595006136435No Hit
GTGCGTGAAGAAGGGAAACAGCTACGGTATCGATATTGTTGAGACCGAGAA10630.5772593485603814No Hit
GGATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGATACTG10540.5723719222790612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5600.30410652417103823No Hit
GCTTTCTTCGAGCCTCTTCGCCTTCCTTCCTCGTCTAACCATGTCGACCTG4930.2677223507434318No Hit
GCGGCAACTGCGACTGCGTTGACAAGAGCCAGTGCGTGAAGAAGGGAAACA4190.22753684576368752No Hit
ACGTCGACGAGGTGATCGTTGCCGCAGAAGCTGCCGAGCATGACGGCAAGT4150.22536465630532296No Hit
GCTTCTTCGAGCCTTTTCGCCTTCCTTCCTCGTCTAACCATGTCGACCTGC3780.2052719038154508No Hit
CCTCGTCTAACCATGTCGACCTGCGGCAACTGCGACTGCGTTGACAAGAGC3490.18952353024230773No Hit
CCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTCGACAGCGCCTCGT2770.15042411999174568No Hit
GGGAAACAGCTACGGTATCGATATTGTTGAGACCGAGAAGAGCTACGTCGA2760.14988107262715455No Hit
AGAAGAGCTACGTCGACGAGGTGATCGTTGCCGCAGAAGCTGCCGAGCATG2400.13033136750187352No Hit
GCACCGACTGCAAGTGTGGCAACTGAGAAGCACTTGTGTCACTACCACTAA2360.12815917804350896No Hit
GGTAGGTAGGTAGGAGATGATTCATCAACCTTACAAGTTAGTTTCTAGAAG2340.12707308331432668No Hit
GACAAGAGCCAGTGCGTGAAGAAGGGAAACAGCTACGGTATCGATATTGTT2240.12164260966841527No Hit
GCCCGAATTATTAGAGTACATAGCCACACACCTTCTTCTACTTCCTTTTTT2120.11512604129332162No Hit
GGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA2100.11403994656413934No Hit
AGTAGAAGAAGGTGTGTGGCTATGTACTCTAATAATTCGGGCAGGCTGATA1980.10752337818904566No Hit
ACTGCAAGTGTGGCAACTGAGAAGCACTTGTGTCACTACCACTAAATAAAA1980.10752337818904566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)