FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11223
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA3312.9493005435266864RNA PCR Primer, Index 10 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT2612.3255813953488373RNA PCR Primer, Index 10 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC1681.4969259556268377TruSeq Adapter, Index 10 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC1050.9355787222667735TruSeq Adapter, Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC860.7662835249042145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.4633342243606879No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.37423148890670943No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA320.28512875345273103TruSeq Adapter, Index 10 (96% over 28bp)
CCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAA270.24057738572574178RNA PCR Primer, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT260.23166711218034394TruSeq Adapter, Index 10 (96% over 27bp)
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG250.22275683863494608TruSeq Adapter, Index 10 (96% over 28bp)
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT210.18711574445335472Illumina Paired End PCR Primer 2 (96% over 25bp)
GTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAAC170.15147465027176332No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG130.11583355609017197RNA PCR Primer, Index 10 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)