FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6426
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT4286.660441954559601RNA PCR Primer, Index 46 (95% over 21bp)
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC3124.855275443510738TruSeq Adapter, Index 4 (95% over 24bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAA2904.5129162776221605RNA PCR Primer, Index 46 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC2123.299097416744476RNA PCR Primer, Index 46 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1983.081232492997199No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT420.6535947712418301TruSeq Adapter, Index 18 (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.6224712107065048No Hit
CCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAA340.5291005291005291RNA PCR Primer, Index 46 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTT310.4824151882975412RNA PCR Primer, Index 46 (96% over 27bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA280.4357298474945534RNA PCR Primer, Index 46 (96% over 28bp)
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG280.4357298474945534TruSeq Adapter, Index 4 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.3734827264239029No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG220.3423591658885776RNA PCR Primer, Index 46 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC200.3112356053532524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC170.26455026455026454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC120.18674136321195145No Hit
TACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT110.1711795829442888TruSeq Adapter, Index 4 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC100.1556178026766262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC100.1556178026766262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC100.1556178026766262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC90.1400560224089636No Hit
CTCCATGGGGAACTTTACTTTCATTACGCCTATAAGTTTTAACACTCATTG80.12449424214130098No Hit
AATCTGGGTACGGATGAAATCAACCACTGACTCTGGCACGTCAGTAATTTC80.12449424214130098No Hit
CCCCTCGGTCGTTTCGCTGTCCGTGACATGCGCCAGACCGTCGCCGTCGGT80.12449424214130098No Hit
TCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGGG70.10893246187363835No Hit
GCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATA70.10893246187363835No Hit
CAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAG70.10893246187363835No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT70.10893246187363835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)