FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11791
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA5084.308370791281486RNA PCR Primer, Index 11 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT3342.8326689848189295RNA PCR Primer, Index 11 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC2331.976083453481469TruSeq Adapter, Index 11 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1471.2467135951149182No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC1381.1703841913323723TruSeq Adapter, Index 11 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.4410143329658214No Hit
CCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA460.39012806377745735RNA PCR Primer, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA440.3731659740480027TruSeq Adapter, Index 11 (96% over 28bp)
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT340.2883555254007294RNA PCR Primer, Index 17 (96% over 25bp)
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG310.2629123908065474TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT290.2459503010770927TruSeq Adapter, Index 11 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.22898821134763803No Hit
GCTCAAAAACACTGTGTTTGGTGTCATTGGGTCAAGGGTTGGGGATACACA210.17810194215927402No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG190.16113985242981937RNA PCR Primer, Index 11 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)