FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11952
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.8952476572958501No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT770.6442436412315931RNA PCR Primer, Index 1 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.4350736278447122No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC400.33467202141900937TruSeq Adapter, Index 1 (95% over 22bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAA390.32630522088353414RNA PCR Primer, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC330.2761044176706827No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC310.25937081659973227TruSeq Adapter, Index 1 (96% over 25bp)
GAATTATCCCAGTTTTCTATTTTATTTTATCTACACAGGCATAGTTAGACA180.15060240963855423No Hit
GGTAGAGGGGGTGCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTTTA150.12550200803212852No Hit
ATTAGCTACTCAGTCGAGGCCGACCCGGACACGACGGCAAAAGCGATGCTC130.10876840696117805No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10876840696117805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)