FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133333
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72975.472763681909205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35642.6730066825167063No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.626251565628914No Hit
ACAAACCAACAGGTACGACTCCACTTATATAAACACATTCAAGCACAATAA5560.4170010425026063No Hit
CAAACCAACAGGTACGACTCCACTTATATAAACACATTCAAGCACAATAAA4740.35550088875222186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4500.33750084375210937No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.32700081750204374No Hit
CCATGTATGAAGGCTATATAAGCTTCAACACCGTAATCCTGAAGATATCTA3810.28575071437678595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA3650.27375068437671096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3150.23625059062647655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3140.23550058875147187No Hit
GTTATATTACTGGGTTAGTTCGTATACATAACACATTTTCTTCTTCTTTCC2880.21600054000135002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2770.20775051937629843No Hit
GTTATATTACTGGGTTAGATCGTATACATAACACATTTTTTTCTTCTTTCC2200.16500041250103126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA2170.1627504068760172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2160.16200040500101248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2080.15600039000097501No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA2000.15000037500093752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1890.14175035437588593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1770.13275033187582969No Hit
GTGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCAAATATATTAAAT1740.13050032625081562No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA1730.12975032437581094RNA PCR Primer, Index 10 (96% over 28bp)
TCATACATGGTGGCAGTAAAATGTTTATTGTGCTTGAATGTGTTTATATAA1720.12900032250080626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT1600.12000030000074999No Hit
GTAATATAACAAATTGCAATAACCAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.12000030000074999No Hit
CCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTG1550.11625029062572656No Hit
GAGTGAATGGGGGTTAATTCCGATAACGAACGAGACTCAAATATATTAAAT1460.10950027375068438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT1450.10875027187567969No Hit
AATAATTAGAGGTAGTTTATATTTTTTTTTAAGAATTTACATTGCCAAAAA1420.10650026625066564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)