FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007544683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56872788
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG6674781.1736333376165768TruSeq Adapter, Index 7 (97% over 36bp)
TGTAGGTCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG3830600.6735382833702473TruSeq Adapter, Index 7 (97% over 36bp)
CAAGAGTCTCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG3762770.6616116656704082TruSeq Adapter, Index 7 (97% over 36bp)
CCATAGTCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG1668550.293382838907071TruSeq Adapter, Index 7 (97% over 36bp)
CTCAGCTGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG1170020.20572580334904628TruSeq Adapter, Index 7 (97% over 36bp)
CGGTAGTCTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG1052060.1849847769024441TruSeq Adapter, Index 7 (97% over 36bp)
ATCGACTGTCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG1013730.17824517412439847TruSeq Adapter, Index 7 (97% over 36bp)
CATGACTGTCATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG972220.17094642872088492TruSeq Adapter, Index 7 (97% over 36bp)
CTATTGTCAATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG872120.15334574418964653TruSeq Adapter, Index 7 (97% over 36bp)
ACTCACTGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG855880.15049024851744564TruSeq Adapter, Index 7 (97% over 36bp)
ATCAAGTCTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG829830.14590985059498052TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)