FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007544684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56872788
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGACCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC7169941.260697822656417Illumina Single End PCR Primer 1 (100% over 38bp)
TGTAGGACCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC4379070.7699763197823184Illumina Single End PCR Primer 1 (100% over 38bp)
CAAGAGACTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC3925210.6901736556329893Illumina Single End PCR Primer 1 (100% over 38bp)
GTGAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC3072320.5402091418482948Illumina Single End PCR Primer 1 (100% over 38bp)
CCATAGACTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1733080.3047292142597265Illumina Single End PCR Primer 1 (100% over 38bp)
CGGTAGACTACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1246720.21921204214570947Illumina Single End PCR Primer 1 (100% over 38bp)
CTCAGCAGCTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1229550.21619302363021137Illumina Single End PCR Primer 1 (100% over 38bp)
ATCGACAGTCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1095610.19264221757512573Illumina Single End PCR Primer 1 (100% over 38bp)
CATGACAGTCATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1006110.17690534179544706Illumina Single End PCR Primer 1 (100% over 38bp)
CTATTGACAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC923330.16235005043185152Illumina Single End PCR Primer 1 (100% over 38bp)
ACTCACAGTGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC904140.15897585326747127Illumina Single End PCR Primer 1 (100% over 38bp)
ATCAAGACTTGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC889430.15638937904714642Illumina Single End PCR Primer 1 (100% over 38bp)
TGACAGACTGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC624390.10978712701758178Illumina Single End PCR Primer 1 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)