FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6577
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA1211.8397445643910597RNA PCR Primer, Index 11 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1141.7333130606659573RNA PCR Primer, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1001.5204500532157519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT881.3379960468298617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC851.292382545233389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC630.9578835335259236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC610.9274745324616087No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC580.8818610308651361TruSeq Adapter, Index 11 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC500.7602250266078759TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC450.6842025239470884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC450.6842025239470884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC380.5777710202219857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC320.48654401702904065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.4561350159647256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC260.39531701383609547No Hit
GGTATCAACGCAGAGTGAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA220.33449901170746543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC210.3192945111753079No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC180.27368100957883534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC180.27368100957883534No Hit
GTATCAACGCAGAGTGAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.27368100957883534No Hit
GTATCAACGCAGAGTGAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA160.24327200851452033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCT130.19765850691804773No Hit
GTATCAACGCAGAGTGAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA120.18245400638589024No Hit
GGTATCAACGCAGAGTGAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA120.18245400638589024No Hit
CCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA110.16724950585373272RNA PCR Primer, Index 11 (96% over 28bp)
GGTATCAACGCAGAGTGAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA110.16724950585373272No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA100.1520450053215752No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT100.1520450053215752TruSeq Adapter, Index 11 (96% over 27bp)
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT100.1520450053215752RNA PCR Primer, Index 17 (96% over 25bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA100.1520450053215752TruSeq Adapter, Index 11 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC90.13684050478941767No Hit
GGTATCAACGCAGAGTGAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.13684050478941767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCT90.13684050478941767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCT90.13684050478941767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCTT90.13684050478941767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCTT80.12163600425726016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC80.12163600425726016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCTT80.12163600425726016No Hit
GTATCAACGCAGAGTGAATGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.12163600425726016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCT70.10643150372510264No Hit
AGTTTCACGCCTGGGCAACACTATCATAACTATGAGACATCATCATAACTA70.10643150372510264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCT70.10643150372510264No Hit
ATATCGACTTCGCGTCCAAATACGGCATCCCCGGCGTGCTGGTCGAAGGAT70.10643150372510264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)