FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8853
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA2663.0046311984637972RNA PCR Primer, Index 10 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT2312.609284988139614RNA PCR Primer, Index 10 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC1191.3441771151022253TruSeq Adapter, Index 10 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC971.0956737828984524TruSeq Adapter, Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC941.0617869648706653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.7906924206483679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.36145939229639673No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA240.27109454422229756TruSeq Adapter, Index 10 (96% over 28bp)
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT180.20332090816672313Illumina Paired End PCR Primer 2 (96% over 25bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT150.16943409013893596TruSeq Adapter, Index 10 (96% over 27bp)
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG150.16943409013893596TruSeq Adapter, Index 10 (96% over 28bp)
CCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAA140.15813848412967355RNA PCR Primer, Index 10 (96% over 28bp)
GTAAAGGGGAAGGTTATCGCTTAGACCGCAGGAGCAAAGTTTCATCCATAC140.15813848412967355No Hit
CTCCATGGGGAACTTTACTTTCATTACGCCTATAAGTTTTAACACTCATTG130.14684287812041116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC130.14684287812041116No Hit
GCTTCTCACTGGTCTCTGCGACCCACACCAGCTCGGACAGTAAATGTCGTC120.13554727211114878No Hit
CCTTCTTCTTCTCTTTCGAACGCTCTACTGCAGGTAGAAGGCGAAGAAGGA110.12425166610188637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC110.12425166610188637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC100.11295606009262397No Hit
TACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT90.10166045408336156TruSeq Adapter, Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG90.10166045408336156RNA PCR Primer, Index 10 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC90.10166045408336156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)