FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26393
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT1760.6668434812260826RNA PCR Primer, Index 17 (95% over 21bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC1240.4698215435911037TruSeq Adapter, Index 12 (95% over 24bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAA1100.41677717576630163RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC930.35236615769332774RNA PCR Primer, Index 17 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC920.3485772742772705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.303110673284583No Hit
CTCCATGGGGAACTTTACTTTCATTACGCCTATAAGTTTTAACACTCATTG410.1553442200583488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.12124426931383321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)