FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102008
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA6090.5970119990588973RNA PCR Primer, Index 11 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT3010.29507489608658144RNA PCR Primer, Index 11 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC2560.2509607089640028TruSeq Adapter, Index 11 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC1810.17743706375970514TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1100.10783467963296997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)