FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007544735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39103870
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC5766831.474746617150681Illumina Single End PCR Primer 1 (100% over 38bp)
AGAAGGACCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC5135021.3131743737896018Illumina Single End PCR Primer 1 (100% over 38bp)
TGTAGGACCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2072270.5299398755161573Illumina Single End PCR Primer 1 (100% over 38bp)
CGGTAGACTACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1199830.3068315233249292Illumina Single End PCR Primer 1 (100% over 38bp)
CAAGAGACTCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1104970.2825730547897177Illumina Single End PCR Primer 1 (100% over 38bp)
ATCAAGACTTGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC991900.2536577581707386Illumina Single End PCR Primer 1 (100% over 38bp)
CCATAGACTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC766740.19607777951389468Illumina Single End PCR Primer 1 (100% over 38bp)
GTAAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC763020.19512646702231773Illumina Single End PCR Primer 1 (100% over 38bp)
ATGAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC760550.19449481598624382Illumina Single End PCR Primer 1 (100% over 38bp)
GTTGAGACTCAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC738350.18881762853651057Illumina Single End PCR Primer 1 (100% over 38bp)
ATCGACAGTCGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC600070.15345539968294697Illumina Single End PCR Primer 1 (100% over 38bp)
ACAACCAGGTTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC524690.13417853527029422Illumina Single End PCR Primer 1 (100% over 38bp)
CATGACAGTCATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC523830.13395860818890815Illumina Single End PCR Primer 1 (100% over 38bp)
CTCAGCAGCTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC496520.12697464470907865Illumina Single End PCR Primer 1 (100% over 38bp)
ACTCACAGTGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC483690.1236936395297959Illumina Single End PCR Primer 1 (100% over 38bp)
TGCTGCAGCAGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC471670.12061977497367907Illumina Single End PCR Primer 1 (100% over 38bp)
GTGAAGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC420260.10747273863175179Illumina Single End PCR Primer 1 (100% over 38bp)
ACAGACAGTCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC419620.10730907196653426Illumina Single End PCR Primer 1 (100% over 38bp)
CTATTGACAATAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC414950.10611481676877507Illumina Single End PCR Primer 1 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)