FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13885
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT4993.5938062657544108RNA PCR Primer, Index 26 (100% over 22bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA4643.3417356859920777RNA PCR Primer, Index 26 (100% over 29bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC3452.484695714800144RNA PCR Primer, Index 26 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC2071.4908174288800864RNA PCR Primer, Index 26 (100% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1851.3323730644580483No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA640.4609290601368383RNA PCR Primer, Index 26 (100% over 29bp)
CCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAA450.32409074540871446RNA PCR Primer, Index 26 (100% over 29bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT450.32409074540871446RNA PCR Primer, Index 26 (96% over 29bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT420.30248469571480013RNA PCR Primer, Index 26 (100% over 28bp)
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG330.23766654663305728RNA PCR Primer, Index 26 (96% over 31bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG300.21606049693914295RNA PCR Primer, Index 26 (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.14404033129276198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)