FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4349
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.7587951253161646No Hit
CTCCATGGGGAACTTTACTTTCATTACGCCTATAAGTTTTAACACTCATTG160.36790066681995864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.29891929179121635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC120.27592550011496897No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCG110.2529317084387216No Hit
GTTAATTTGCCGAGTTCCTTCTCCAGAGTTCTCTCAAGCGCCTTAGAATAC110.2529317084387216No Hit
CTCTTCGGGGTTGTAGGACTGCAATGTGGACTCAAAGATTATAGAAGAATG100.2299379167624741No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC100.2299379167624741No Hit
CCTCACAGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGG90.20694412508622673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC90.20694412508622673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC90.20694412508622673No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.20694412508622673No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC80.18395033340997932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC80.18395033340997932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC80.18395033340997932No Hit
GAGTGAATGGGGGTTTTCAAGCCAAGCGCCGCCCGCTAATCCCTCGCCTAT80.18395033340997932No Hit
CATAAACAACAACCAAATCAACCAAAATGGAGAAAGCCAAGTCTGCCGTTT70.1609565417337319No Hit
CCTATTTATTGCAGCCCGTTCATGCTCAATTGTTCATATCACATTACTAGG70.1609565417337319No Hit
TCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTGACGTATT70.1609565417337319No Hit
CTGCTTCCCATTCACTCTGCGTTGATACCACTGCTTCCCATTCACTCTGCG70.1609565417337319Clontech Universal Primer Mix Long (96% over 26bp)
ATTAAGTCATATAAGTTCTAGTGGAATGCTCTGGGAAGTGCAACCATAGTG70.1609565417337319No Hit
GCTCTAGTCATAAGTATAATATACCCCATAAAAATCACCCCAAGAACACTA70.1609565417337319No Hit
GATTTTTATGGGGTATATTATACTTATGACTAGAGCTGGTAGTTTCGAAGC70.1609565417337319No Hit
GAGTGAATGGGAAGCAGTGGTATCAACGCAGAGTGAATGGGAAGCAGTGGT70.1609565417337319Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC70.1609565417337319No Hit
GCTCGCCGTAAGCGCAAGGTTTTCTACGCAACGTTCATCGGCGTAGAGTGA60.13796275005748448No Hit
GTCTTGTGGGAGACCTTTAACCAAATACCCAGTGACTTTTAGTGTTAGCTC60.13796275005748448No Hit
GCGATGTGGGGACGGATTCTAATAGGTCATCCAGTTATTGGATTCTGGTTT60.13796275005748448No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA60.13796275005748448RNA PCR Primer, Index 11 (96% over 28bp)
GAGTGAATGGGGGGTCAAGTGACTAAGAGCATGTGGTGGATGCCTTGGCGA60.13796275005748448No Hit
CCCACTTATGCTACGCCTCTCATGTCGTTATACAATATCAAGCTAAAGTCA60.13796275005748448No Hit
ACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCG60.13796275005748448No Hit
GAGTGAATGGGGGGATTCATCCTGAATACAAGCGAAAGTCTCGCTTCAAGT60.13796275005748448No Hit
CATAAAAATCACCCCAAGAACACTACTAGGTAGTGCCCCTGAAAATTTCAT60.13796275005748448No Hit
CATATTCAGACAAGGTTTCACGTGCCCCGCCCTACTCGACATCATCATATA60.13796275005748448No Hit
CGTAATATAGCCGGCAGTGAGGCTTGGGGTGCTAAGGTCCCAGGCCGAGAG60.13796275005748448No Hit
GTGTGGATATGGTGAACAAGGCGACGAACTATGTAACGATGACGCTGGCCG50.11496895838123705No Hit
GCTAAGTCAGTCAGTTGTAAGACAAATCACCCTCAAAAGGACTCAACGGGT50.11496895838123705No Hit
GATCCGTTGCAAGGCGCTATGTAAGCCTAGATTACCTGGAAGTCCCCTCGG50.11496895838123705No Hit
CCCACAAGACTGGTATTCTCTTTGCCTGAAGAAACAAGGCATTGAATCTCT50.11496895838123705No Hit
GCATCCTATATCCTCTTTTGTCGAGTTCTTTTCCGACGTCTTAGCGACCTA50.11496895838123705No Hit
GTCTGAATCCAGCGGCACCATCCGCTAAGGCTAATACTCCTGAGAGACCGA50.11496895838123705No Hit
CCTTTAGCATCCTTTGTAGTTTTCTCCATTGATCCTACCGTAGCATCTTTG50.11496895838123705No Hit
CCTTAGCACCTTCAATTGAACTCATTGCTTTTTGTTGAGCTTCAGCTCTGT50.11496895838123705No Hit
GGTATGTCGGTCGGTTATGTCAGGCAGGCAAAGGTCTTCCAACCTTACGCG50.11496895838123705No Hit
ACTAGTGACGGTACATTCAAGTGTCCACGTTCAATTAAGTAACAAGGACTT50.11496895838123705No Hit
CCCGATAGCGGGGTCTTTACGAATTGCTGCTCTCGAAGTCGCAGAAACAGG50.11496895838123705No Hit
AGATTACCTGGAAGTCCCCTCGGGGATGGATAGTATGCTAGTACTATAGCT50.11496895838123705No Hit
GAGTGAATGGAGCAGTTCACCCATTTTCTGGCCACGTACGCTCTGCGATAG50.11496895838123705No Hit
GCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA50.11496895838123705No Hit
GGAAGTCCCCTCGGGGATGGATAGTATGCTAGTACTATAGCTCTTTGTAAC50.11496895838123705No Hit
AGTAAACGGCGGCCGTAACAATAACGGTCCTAAGGTAGCGAAATTCCTTGT50.11496895838123705No Hit
ACCATTGGTAGAGTGGACAACATTACTACACCACTTGCTAAAAAGGCTCAA50.11496895838123705No Hit
AGTGTGTAAGAGCGCATGGTGGATGCCTTGGCATCAGGAGCCGATGAAGGA50.11496895838123705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC50.11496895838123705No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAA50.11496895838123705RNA PCR Primer, Index 23 (96% over 30bp)
CCAGCAGCCACTGGCATCGGAAAGAAACATCAAGAAGTGCTTGAACTATTC50.11496895838123705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC50.11496895838123705No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGATTCCCCAGCACG50.11496895838123705No Hit
CCATGGGGAACTTTACTTTCATTACGCCTATAAGTTTTAACACTCATTGAC50.11496895838123705No Hit
GCGATAAGCTTCGGGGAGCTGGCAAATAAGCTTTGATCCGGAGATTTCCGA50.11496895838123705No Hit
CCTACTCATCGGGGCTTGGTCCTTGCCCCGACGGCCGGGTATAGGTCGCGC50.11496895838123705No Hit
CAAAATAAGCTAGGGAAGAAAACCCAAAACAAAGAAGATATGACATCCAAG50.11496895838123705No Hit
ACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGA50.11496895838123705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC50.11496895838123705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCC50.11496895838123705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)