FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258074
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTGA19010.7366104295667134No Hit
GCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTGCTATCACTAA13980.5417050923378566No Hit
CCTAATACGTAGTTAAGTTGAACAAACGAGGTGAGACTTCAATAACAGCCA10410.40337267605415505No Hit
GCGATAAGCTTCGGGGAGCTGGCAAATTAGCTTTGATCCGGAGATTTCCGA8270.32045072343591374No Hit
GCATTGCTGCAGGGTACGCTTTATCCCGAAGTTACAGCGTCAATTTGCCTA7930.3072762075993707No Hit
GGTTGAGCCCGGGGATTTCACAACTGTCTTACATCACCGCCTGCGCACGCT7300.28286460472577635No Hit
CATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAGT7280.2820896332059797No Hit
ACTGATCGCAGGCTTGGTGGGCCTCTACCCCACCAACTACCTAATCAGACG5390.2088548245851965No Hit
GCGATCAGTAGCTGATGTGAGAGCATGATCAGCCACACGGGCACTGAGACA5310.2057549385060099No Hit
GGCCATAGCGGGTGATAGCCCCGTACGTGAAATGACCTGTGTGGTACTGAG5170.20033013786743334No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC5140.1991676805877384No Hit
GCCTACGCACCCTTTAAACCCAGTGAATCCGGATAACGCTTGCACCCTCCG5100.1976177375481451No Hit
GCAGCAATGCGGGGTCTCAGTAAAATGGTTCAACCAACTGTTTAACAAAAA4940.1914179653897719No Hit
GCGTAGGCGGGTATGTAAGTCAGTGGTGAAATACCGGAGCTTAACTTCGGA4760.1844432217116021No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC4720.1828932786720088No Hit
TCTACCTGTGTCGGTTTGCGGTACTGGCTGCTATACTCGCTTTTCTTGGCA4640.1797933925928222No Hit
CCGGTGAGCGGGGGATAGCCCAGAGAAATTTGGATTAATACCCCATACTAT4560.17669350651363563No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCACAGGG4430.17165619163495743No Hit
GGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGC4370.16933127707556747No Hit
CCTTCCTCACGCCTTACGGCGGCAGTTTCAGTAGAGTTCCCAGCTTTACCT4310.16700636251617754No Hit
GGTAGTCCACGCCCTAAACGATGATTACTCGACATTAGCGATACATAGTTA4040.15654424699892278No Hit
GTGGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTC4000.1549943039593295No Hit
CACAGGTAGACGGGATGAGTATTCTAAGGCGCTCGGGTGAGCCGTGGAGAA3930.15228190364004124No Hit
TCCGGTGAGCGGGGGATAGCCCAGAGAAATTTGGATTAATACCCCATACTA3830.148407046041058No Hit
CCATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAG3780.14646961724156637No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGG3740.14491967420197308No Hit
GGGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAGA3650.14143230236288817No Hit
GCCGTTTACCGGGGCTTCAGTCAGGAGCTTTGGTTTAACCCTAACCCCCTT3630.1406573308430915No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAGT3600.13949487356339654No Hit
CCCAATCGTGGGGGATAACGCAGCGAAAGCTGTGCTAATACCGCATACGAT3590.1391073878034982No Hit
CGTATTAGGGGCCTTAGTTGCTAATCTGGGTTATTTCCCTCTCGGCCACGG3550.1375574447639049No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG3450.13368258716492168No Hit
GCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCCGCT3420.1325201298852267No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC3320.12864527228624348No Hit
GCTTTTGCGGGGTTGTAGGACTACATTTAGGCGTTATCAGGAAGCTGAATT3300.12787030076644684No Hit
CCAACATGGCTCACGCCACACCTTCAACGCCGGTTTCAATGCTCCCCTACC3290.1274828150065485No Hit
GCGACTGATCGCCCTATTCGGACTCGATTTCTCTACGGCTTCCCTATTCGG3270.12670784348675185No Hit
GCTTATTGATGGGTGTGCGTCTGATTAGGTAGTTGGTGGGGTAGAGGCCCA3260.12632035772685354No Hit
GCTCATGGATAGATCACAAAGTTTCGCGTCTGCCCCAACTGACTATTCGCC3260.12632035772685354No Hit
ACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTG3230.12515790044715858No Hit
GCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTTC3200.12399544316746358No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA3090.11973309980858203No Hit
CCTACCTGTGTCGGTTTCAGGTACAGGTGATTCTGGTGTTGGATCTTGCTT2990.1158582422095988No Hit
CCCGTAGGAGTCGGGCCCGTGTCTCAGTGCCCGTGTGGCTGATCATGCTCT2920.11314584189031053No Hit
AATCGGATCAGGGTTAGTCTGGTCCTTAGGAGTACCCGAAAGGGGAATCTG2840.11004595581112393No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGT2840.11004595581112393No Hit
CACAGGTAGGTAGGTAGAGAATACTAAGGGGCGCGAGATAACTCTCTCTAA2740.1061710982121407No Hit
GATCCGATTAACGTTGATCAGGAAACCTTAGACTTTCGGCGAAGGAGTTTT2620.10152126909336082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)