FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707788
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTAGGATGGTTATAGTTACCACCGCCGTTTACTGGCGCTTAAGTTCTC170820.6308470234745113No Hit
GCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGGACCTACTCC103540.38237853184961307No Hit
GGTAATATTTAGGCTTAGCGGGTGGTCCCGCCAGATTCACACAGGATTTCT86620.3198921038131493No Hit
GTCTAATACTGGATATGACTCAAGCTCGCATGGGTTTGGGTGGAAAGATTT85750.31667914918006873No Hit
GCTAACCGGTCCCCTTAACGTTCCAGCACCGGGCAGGCGTCAGTCCGTATA82520.30475059347334427No Hit
CTTGAATGGCGGGCCGTAGTGGGTGGTAGCCCCGTAGGCGAAATTGCGTGG71140.26272366965212934No Hit
CCGTAACTTCGGGAGAAGGGGGGCCATGATCGTGATCCGGCTGTGCCTGGT67890.25072125291935704No Hit
ACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCG60690.22413128354213846No Hit
GCGAGATTCGTGTCTCGTCACCCGCATCTGAACTCATAGGGTGTGTGGAGG56930.21024541064514654No Hit
GTTATCCCCGGGGTACCTTTTATCCGTTGAGCGACCGCGTTTCCACACACC56770.20965452243676386No Hit
AAATATTACCTGGTGACCGATAGCGGACAAGTACCGTGAGGGAATGGTGAA55470.204853555743655No Hit
GACGAAAGAGGTTTACAACCCGAAGGCCTTCATCCCTCACGCGGCGTCGCT54040.19957249238123517No Hit
GCTATCACGGAGTTTGATTGGCCTTTCACCCCTACCCACAGCTCATCCCCT53710.19835378545144597No Hit
GGTAGTCCATGCCGTAAACGTTGGGCGCTAGGTGTGGGGCTCATTCCACGA52350.19333123568019359No Hit
CCTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCAGTCTCCCCTAC51730.19104154387271086No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTTCAGG48690.17981466791344078No Hit
GGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGC47590.17575231148081016No Hit
CCCCACAACCCCGAACACACAACACCTGCCGGCTATCACATGCGCCCGGTT47370.17493984019428405No Hit
CCCGGGGTACTTTTCACCATTCCCTCACGGTACTTGTCCGCTATCGGTCAC46780.17276093992587307No Hit
CCTCCCGGGGTACTTTTCACCATTCCCTCACGGTACTTGTCCGCTATCGGT46530.1718376771002752No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC45730.16888323605836203No Hit
CCGAATGACCAAGGGTTCCAGGGTCAAGTTAATCTGCCCTGGGTAAGTCGG45530.16814462579788372No Hit
CTCAGGTGGTCAGGTAGAGAATACTAAGGCGATCGAGTGAATCGTGGTTAA45380.16759066810252501No Hit
CACACCTAGCGCCCAACGTTTACGGCATGGACTACCAGGGTATCTAATCCT43640.16116475883636386No Hit
CCCCGGGAGGGGAGTGAAATAGTTCCTGAAACCGTGTGTCTACAATCCGTC43570.16090624524519645No Hit
GATCTGGTAGTAGGTAAGTGATGGAGTGACGCAGGAAGGTAGCCGCCGCGT42080.15540359880463317No Hit
GTTGTGGGGTGCGTTTTTATCCAGGCTGCCGTCTGGGTGTGGAGTGAGAAA41910.1547757800832266No Hit
GATATCAGGAGGAACACCGATGGCGAAGGCAGGTCTCTGGGCCATAACTGA40990.15137817288502645No Hit
CCCTCAGCCTGGTTGGCAATCAGGTGTTGAGTGTAAGTGCACAAGGGAGCT40630.1500486744161655No Hit
GGTCTCTGCGGCCACTCACGCTCACCAGGCACAGCCGGATCACGATCATGG40630.1500486744161655No Hit
GGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCT40120.14816521825194587No Hit
ATGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTC40080.1480174961998502No Hit
CCCAGGCGGGGCGCTTAATGCGTTAGCTGCGGCACAGAACTCGTGGAATGA40020.14779591312170673No Hit
CCCGTAACTTCGGGAGAAGGGGGGCCATGATCGTGATCCGGCTGTGCCTGG39990.14768512158263497No Hit
GCAGAGACCAGGGAGAAGCGACTGTTTACTAAAAACACAGGTCCGTGCGAA39500.14587552644446314No Hit
CCCTTCTCCCGAAGTTACGGGGGCATTTTGCCGAATTCCTTAACCACGATT38480.14210861411602385No Hit
GGGGATAACAGGCTGATCTTGCCCAAGAGTCCATATCGACGGCATGGTTTG38210.14111149026437816No Hit
AGTAGCACGGGGCCCGAGAAATCCTGTGTGAATCTGGCGGGACCACCCGCT36190.13365152663354737No Hit
GCCATTCAAGACGGTTCGACTACCACGCAGATTTCTCACTCCACACCCAGA36120.13339301304238No Hit
GGGAGAAGGGGGGCCATGATCGTGATCCGGCTGTGCCTGGTGAGCGTGAGT34570.1276687835236732No Hit
ACTACCAGATCGGGTCACGCGCTCCACCAATCCGACACACGCCAAAGGCGT34390.12700403428924273No Hit
CCCCGTGTGGGGGAAGAGGGTGATCCTATGCTGTCGAGAAAAACTTCTAGC33510.12375414914313823No Hit
GCAGGTGTTGTGTGTTCGGGGTTGTGGGGTGCGTTTTTATCCAGGCTGCCG33480.12364335760406649No Hit
CTTTCGTCAGGGAAGAAGCGAAAGTGACGGTACCTGAAGAAGAAGCACCGG33020.1219445540049664No Hit
AAGTTACGGGGGCATTTTGCCGAATTCCTTAACCACGATTCACTCGATCGC32740.1209104996402968No Hit
GGGCTTAACTCTGGGCGTGCGGTGGGTACGGGCAGGCTAGAGTGTGGTAGG32550.12020881989284243No Hit
GCCCGTACCCACCGCACGCCCAGAGTTAAGCCCTAGGTTTTCACGGCAGAC31930.11791912808535972No Hit
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCC31430.11607260243416398No Hit
GAGTTAAGCCCTAGGTTTTCACGGCAGACGCGACAAACCGCCTACAAGCTC30760.11359825806156168No Hit
CCGCACGCCCAGAGTTAAGCCCTAGGTTTTCACGGCAGACGCGACAAACCG30740.11352439703551387No Hit
AAGACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGAGTT30370.11215796805362903No Hit
CCCATGGCCAGGGTGAAGCGCCGGTAAGACGGCGTGGAGGCCCGAACCCAC30100.11116084420198331No Hit
GACGGCAGCCTGGATAAAAACGCACCCCACAACCCCGAACACACAACACCT29550.109129665985668No Hit
GGCCATGGGTAGATCACTCCGCTTCGGGTCTAGACCCCGCGACTCAACACG29340.1083541252121658No Hit
GCCTGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCT29200.10783709802983099No Hit
GGGAGAAGCGACTGTTTACTAAAAACACAGGTCCGTGCGAAGACGTAAGTC29180.10776323700378315No Hit
GGTCATTCGGCGGGCAAGTTTCTCACTTGCCATTCGCTACTCATGCCTGCA29140.1076155149516875No Hit
GGTAAATCCGGGTGTCATGTGCCCCAGGTGTGATGGTGACCCCGTGTGGGG29130.10757858443866358No Hit
ACAATGTAGCGGGGCTCAAGCACACCACCGAAGCTGTGGCAATCACACTGT29000.10709848776935268No Hit
GCCTTCGCCATCGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACC28350.10469800442279825No Hit
GCTACATTGTCGGCGCGGAATCACTTGACCAGTGAGCTATTACGCACTCTT28260.104365629805583No Hit
CCACACACCCTATGAGTTCAGATGCGGGTGACGAGACACGAATCTCGCCGG28230.10425483826651126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)