FastQCFastQC Report
Tue 7 May 2024
EGAF00007544799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104307
Total Bases5.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTG12011.1514088220349545No Hit
GCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTGCTATCACTA8670.8312002070810204No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC7860.7535448244125514No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT7110.6816416923121171No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC4440.42566654203457105No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA3800.36430920264220046No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGC3600.3451350340820846No Hit
CATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAG3350.3211673233819399No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTA3220.30870411381786456No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC3190.3058279885338472No Hit
GGTTGAGCCCGGGGATTTCACAACTGTCTTACATCACCGCCTGCGCACGC3060.2933647789697719No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGC3010.288571236829743No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA2990.2866538199737314No Hit
GGCCATAGCGGGTGATAGCCCCGTACGTGAAATGACCTGTGTGGTACTGA2860.2741906104096561No Hit
CCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCC2730.2617274008455808No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC2550.24447064914147662No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAG2130.2042048951652334No Hit
GCGATAAGCTTCGGGGAGCTGGCAAATTAGCTTTGATCCGGAGATTTCCG2090.20037006145321024No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2070.19845264459719866No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT2040.1955765193131813No Hit
CCTAATACGTAGTTAAGTTGAACAAACGAGGTGAGACTTCAATAACAGCC1960.18790685188913497No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT1930.1850307266051176No Hit
GGGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAG1870.17927847603708283No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGA1870.17927847603708283No Hit
GTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCAT1630.15626947376494385No Hit
CCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCC1610.15435205690893228No Hit
CCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCC1580.1514759316249149No Hit
GGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTT1550.14859980634089753No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTAC1530.14668238948488596No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGG1510.1447649726288744No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACA1510.1447649726288744No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGC1440.13805401363283384No Hit
CCCAATCGTGGGGGATAACGCAGCGAAAGCTGTGCTAATACCGCATACGA1430.13709530520482804No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTC1420.13613659677682224No Hit
ATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGC1400.13421917992081067No Hit
CCATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCA1400.13421917992081067No Hit
GCAGCAATGCGGGGTCTCAGTAAAATGGTTCAACCAACTGTTTAACAAAA1380.1323017630647991No Hit
GGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATG1360.13038434620878753No Hit
ACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCT1360.13038434620878753No Hit
GGCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTG1350.12942563778078173No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAA1320.12654951249676435No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACG1270.12175597035673541No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAG1260.12079726192872962No Hit
GCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGT1230.11792113664471225No Hit
GCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCCGC1220.11696242821670647No Hit
GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCAT1210.11600371978870065No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCG1200.11504501136069487No Hit
CCACTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCC1190.11408630293268908No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTC1180.11312759450468328No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAG1160.11121017764867171No Hit
GGAAATAGCTGGTTCTCTCCGAAAACTATTTAGGTAGTGCCTCAAGTATT1110.10641663550864276No Hit
GATCAGTCGCTGGGTGTAGACCCGAAACCAAGTGATCTATCCATGGCCAG1100.10545792708063696No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGC1070.1025818017966196No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAA1070.1025818017966196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)