FastQCFastQC Report
Wed 8 May 2024
EGAF00007544806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566347
Total Bases28.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTG77461.3677127273561969No Hit
GCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTGCTATCACTA55520.9803177204081597No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACA30930.5461316118916495No Hit
GGTTGAGCCCGGGGATTTCACAACTGTCTTACATCACCGCCTGCGCACGC30700.5420704974158952No Hit
GCGATAAGCTTCGGGGAGCTGGCAAATTAGCTTTGATCCGGAGATTTCCG30400.5367733915779549No Hit
CATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAG29420.5194695125073497No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAG26980.47638638502543496No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGG23650.4175885102242971No Hit
GGGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAG19560.3453713006337104No Hit
GGCCATAGCGGGTGATAGCCCCGTACGTGAAATGACCTGTGTGGTACTGA18660.3294799831198894No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAA17860.3153543675520485No Hit
GCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTT17510.30917441074111807No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTC16790.29646135673006124No Hit
GGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATG15930.28127631999463226No Hit
CCATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCA14850.262206738978047No Hit
GTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCAT14090.24878740418859813No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACG12400.21894704130153422No Hit
CCCAATCGTGGGGGATAACGCAGCGAAAGCTGTGCTAATACCGCATACGA12120.2140030758527899No Hit
ACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGGACTTCAATCAA11900.21011853157163365No Hit
CCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGCCG11700.20658712767967344No Hit
ACCTTAGGACCGTTATCGTTACGGCCGCCGTTTACTGGGACTTCAATCAA11220.19811175833896888No Hit
GCGACTGATCGCCCTATTCGGACTCGATTTCTCTACGGCTTCCCTATTCG10790.19051923997125436No Hit
GGTAGTCCACGCCCTAAACGATGTCAACTGGTTGTTGGGTCTTCACTGAC10040.1772764753764035No Hit
ACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCT9880.1744513522628353No Hit
GCTATCACCCGCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGT9040.15961945591660237No Hit
GATCAGTCGCTGGGTGTAGACCCGAAACCAAGTGATCTATCCATGGCCAG8870.15661776260843616No Hit
CACTGGTGCGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCAGGAGA8700.15361606930026997No Hit
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAAT8370.1477892528785356No Hit
GCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCCGC8180.1444344191811734No Hit
GGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAGA8030.14178586626220321No Hit
CGTAGTTAGCCGGTGCTTATTCTTACGGTACCGTCATTAGCCCTCTTTAT7920.13984359412162506No Hit
CCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGCGTGCTTACAAAAAGT7660.13525276906207678No Hit
CCCACCTATCCTACACAGATCTGTTCAAAGTCCAATACAAAGCTACAGTA7300.12889624205654837No Hit
GGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGT7250.1280133910835583No Hit
CCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTAGTTCACTATCGGTCA7060.12465855738619609No Hit
ACCTTTCGGTGGGTTTCCCCATTCGGAAATCTCCGGATCAAAGCTAATTT6690.11812546018606968No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC6430.11353463512652137No Hit
GATCCAGCCATGCCGCGTGCAGGATGAAGGCCTTCGGGTTGTAAACTGCT6390.11282835434812932No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC6310.11141579279134524No Hit
CACCAAGCCTTCGATCTGTAGCTGGTCTGAGAGGACGACCAGCCACACTG6220.10982666103996314No Hit
TCTAATAAAGAGGGCTAATGACGGTACCGTAAGAATAAGCACCGGCTAAC6200.10947352065076711No Hit
GCGCGAATGGAGCGGCCGATGGCAGATTAGGTAGTTGGTGAGGTAAAGGC6130.10823752928858102No Hit
CCGCATATCTACGCATTTCACTGCTACACGCGGAATTCCATCCCCCTCTG6120.10806095909398303No Hit
GTTAGTGATAGCACAAGACATAACGGAACAGCTTGGAAAGGCTGGCCATA6100.107707818704787No Hit
CTTTATTAGAAAGAGCCGTTTCGTTCCGTACAAAAGCAGTTTACAACCCG6100.107707818704787No Hit
CCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTAGTTCACTATCGG5960.10523583598041483No Hit
GAGTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTC5820.10276385325604268No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT5740.10135129169925859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)