FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566347
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTGA77341.3655938850210207No Hit
GCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTGCTATCACTAA55480.9796114396297677No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC30920.5459550416970514No Hit
GGTTGAGCCCGGGGATTTCACAACTGTCTTACATCACCGCCTGCGCACGCT30650.5411876464429051No Hit
GCGATAAGCTTCGGGGAGCTGGCAAATTAGCTTTGATCCGGAGATTTCCGA30390.5365968213833568No Hit
CATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAGT29420.5194695125073497No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAGT26890.47479725327405287No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC21940.3873950069480372No Hit
GGGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAGA19420.3428993179093383No Hit
GGCCATAGCGGGTGATAGCCCCGTACGTGAAATGACCTGTGTGGTACTGAG18590.32824399175770336No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG17770.3137652358006664No Hit
GCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTTC17510.30917441074111807No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCT16690.29469565478408116No Hit
GGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGC15930.28127631999463226No Hit
CCATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAG14830.261853598588851No Hit
GTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATG14080.24861083399400014No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGG12400.21894704130153422No Hit
CCCAATCGTGGGGGATAACGCAGCGAAAGCTGTGCTAATACCGCATACGAT12010.21206080371221178No Hit
ACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG11840.20905911040404557No Hit
CCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGCCGT11690.2064105574850754No Hit
ACCTTAGGACCGTTATCGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG11170.1972289073659788No Hit
GCGACTGATCGCCCTATTCGGACTCGATTTCTCTACGGCTTCCCTATTCGG10780.19034266977665637No Hit
GGTAGTCCACGCCCTAAACGATGTCAACTGGTTGTTGGGTCTTCACTGACT9980.17621705420881545No Hit
ACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTG9860.1740982118736393No Hit
GCTATCACCCGCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTG8970.1583834645544163No Hit
GATCAGTCGCTGGGTGTAGACCCGAAACCAAGTGATCTATCCATGGCCAGG8870.15661776260843616No Hit
CACTGGTGCGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCAGGAGAA8700.15361606930026997No Hit
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATC8360.1476126826839376No Hit
GCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCCGCT8160.14408127879197735No Hit
GGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAGAG8030.14178586626220321No Hit
CGTAGTTAGCCGGTGCTTATTCTTACGGTACCGTCATTAGCCCTCTTTATT7910.13966702392702707No Hit
CCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGCGTGCTTACAAAAAGTA7650.13507619886747876No Hit
CCCACCTATCCTACACAGATCTGTTCAAAGTCCAATACAAAGCTACAGTAA7300.12889624205654837No Hit
GGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTG7250.1280133910835583No Hit
CCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTAGTTCACTATCGGTCAA7060.12465855738619609No Hit
ACCTTTCGGTGGGTTTCCCCATTCGGAAATCTCCGGATCAAAGCTAATTTG6630.11706603901848162No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC6410.11318149473732535No Hit
GATCCAGCCATGCCGCGTGCAGGATGAAGGCCTTCGGGTTGTAAACTGCTT6380.1126517841535313No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG6310.11141579279134524No Hit
CACCAAGCCTTCGATCTGTAGCTGGTCTGAGAGGACGACCAGCCACACTGG6220.10982666103996314No Hit
TCTAATAAAGAGGGCTAATGACGGTACCGTAAGAATAAGCACCGGCTAACT6170.10894381006697307No Hit
CCGCATATCTACGCATTTCACTGCTACACGCGGAATTCCATCCCCCTCTGC6120.10806095909398303No Hit
GTTAGTGATAGCACAAGACATAACGGAACAGCTTGGAAAGGCTGGCCATAG6100.107707818704787No Hit
GCGCGAATGGAGCGGCCGATGGCAGATTAGGTAGTTGGTGAGGTAAAGGCT6090.10753124851018898No Hit
CTTTATTAGAAAGAGCCGTTTCGTTCCGTACAAAAGCAGTTTACAACCCGA6090.10753124851018898No Hit
CCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTAGTTCACTATCGGT5910.10435298500742478No Hit
GAGTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG5810.10258728306144466No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA5730.10117472150466056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)