FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354563
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTGA41961.1834286149429016No Hit
GCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTGCTATCACTAA30580.8624701392982347No Hit
GCGATAAGCTTCGGGGAGCTGGCAAATTAGCTTTGATCCGGAGATTTCCGA16330.4605669514303523No Hit
CATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAGT15020.4236200618789891No Hit
GGTTGAGCCCGGGGATTTCACAACTGTCTTACATCACCGCCTGCGCACGCT14950.4216458006052521No Hit
GGCCATAGCGGGTGATAGCCCCGTACGTGAAATGACCTGTGTGGTACTGAG9850.27780676494727313No Hit
CCTAATACGTAGTTAAGTTGAACAAACGAGGTGAGACTTCAATAACAGCCA9470.2670893466041296No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC9180.25891026418436214No Hit
GGGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAGA8630.243398211319286No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAGT7890.22252744928263807No Hit
CCATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAG7570.21350225488841196No Hit
GGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGC7190.20278483654526838No Hit
CCCAATCGTGGGGGATAACGCAGCGAAAGCTGTGCTAATACCGCATACGAT6600.18614463438091397No Hit
GTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATG6580.18558055973127485No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGG6160.17373499208885304No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC6090.17176073081511609No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG6040.17035054419101825No Hit
CCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGCCGT5670.15991516317269427No Hit
GCATTGCTGCAGGGTACGCTTTATCCCGAAGTTACAGCGTCAATTTGCCTA5580.15737682724931817No Hit
ACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTG5520.15568460330040076No Hit
GCGACTGATCGCCCTATTCGGACTCGATTTCTCTACGGCTTCCCTATTCGG5480.1545564540011225No Hit
GCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTTC5100.14383903565797898No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCT4980.14045458776014416No Hit
GCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCCGCT4830.13622402788785068No Hit
GCTATCACCCGCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTG4750.13396772928929415No Hit
ACCTTAGGACCGTTATCGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG4340.12240419897169193No Hit
GATCAGTCGCTGGGTGTAGACCCGAAACCAAGTGATCTATCCATGGCCAGG4230.11930178839867668No Hit
CGTATTAGGGGCCTTAGTTGCTAATCTGGGTTATTTCCCTCTCGGCCACGG4040.11394307922710492No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC4000.11281492992782664No Hit
CCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGCGTGCTTACAAAAAGTA3900.10999455667963097No Hit
GCGTAGGCGGGTATGTAAGTCAGTGGTGAAATACCGGAGCTTAACTTCGGA3900.10999455667963097No Hit
CCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTAGTTCACTATCGGTCAA3870.10914844470517229No Hit
CGTAGTTAGCCGGTGCTTATTCTTACGGTACCGTCATTAGCCCTCTTTATT3860.10886640738035272No Hit
GCGCGAATGGAGCGGCCGATGGCAGATTAGGTAGTTGGTGAGGTAAAGGCT3790.10689214610661575No Hit
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATC3780.10661010878179618No Hit
GGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTG3690.10407177285842008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)