FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464588
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTGA67461.4520392261530646No Hit
GCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTGCTATCACTAA49031.055343659328265No Hit
GCGATAAGCTTCGGGGAGCTGGCAAATTAGCTTTGATCCGGAGATTTCCGA24330.5236898068826573No Hit
CATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAGT23550.5069007378580592No Hit
GGTTGAGCCCGGGGATTTCACAACTGTCTTACATCACCGCCTGCGCACGCT18460.3973413002488226No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC13830.29768310847460544No Hit
GGCCATAGCGGGTGATAGCCCCGTACGTGAAATGACCTGTGTGGTACTGAG13390.2882123515889347No Hit
GGGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAGA12500.2690555933429189No Hit
CCATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAG12160.26173728120399153No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAGT11930.2567866582864818No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC11390.24516345665406772No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG10010.21545971914900944No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC9770.21029385175682538No Hit
GGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGC9290.1999621169724573No Hit
GCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTTC9180.19759442775103964No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA9180.19759442775103964No Hit
GCGACTGATCGCCCTATTCGGACTCGATTTCTCTACGGCTTCCCTATTCGG9080.1954419830042963No Hit
GCATTGCTGCAGGGTACGCTTTATCCCGAAGTTACAGCGTCAATTTGCCTA8820.18984562666276358No Hit
GTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATG8790.18919989323874056No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGG7880.16961264604337606No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCT7760.16702971234728406No Hit
GCTATCACCCGCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTG7630.16423153417651767No Hit
GATCAGTCGCTGGGTGTAGACCCGAAACCAAGTGATCTATCCATGGCCAGG7590.16337055627782035No Hit
CCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGCCGT7570.1629400673284717No Hit
CCTAATACGTAGTTAAGTTGAACAAACGAGGTGAGACTTCAATAACAGCCA7290.15691322203759028No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA7270.15648273308824162No Hit
ACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTG6980.1502406433226859No Hit
CCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGCGTGCTTACAAAAAGTA6790.14615099830387354No Hit
GGTAGTCCACGCCCTAAACGATGTCAACTGGTTGTTGGGTCTTCACTGACT6750.14529002040517622No Hit
TCTACCTGTGTCGGTTTGCGGTACTGGCTGCTATACTCGCTTTTCTTGGCA6740.14507477593050186No Hit
GCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCCGCT6620.14249184223440983No Hit
CCCAATCGTGGGGGATAACGCAGCGAAAGCTGTGCTAATACCGCATACGAT6540.14076988643701516No Hit
GCGATCAGTAGCTGATGTGAGAGCATGATCAGCCACACGGGCACTGAGACA6390.13754121931690014No Hit
ACTGATCGCAGGCTTGGTGGGCCTCTACCCCACCAACTACCTAATCAGACG5700.12268935056437102No Hit
GCTTATTGATGGGTGTGCGTCTGATTAGGTAGTTGGTGGGGTAGAGGCCCA5500.11838446107088432No Hit
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATC5320.11451006052674627No Hit
ACCTTTCGGTGGGTTTCCCCATTCGGAAATCTCCGGATCAAAGCTAATTTG5320.11451006052674627No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG5270.1134338381533746No Hit
GCCTACGCACCCTTTAAACCCAGTGAATCCGGATAACGCTTGCACCCTCCG5230.11257286025467726No Hit
GGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTG5210.1121423713053286No Hit
TCTAATAAAGAGGGCTAATGACGGTACCGTAAGAATAAGCACCGGCTAACT5120.11020517103325958No Hit
GGAAATAGCTGGTTCTCTCCGAAAACTATTTAGGTAGTGCCTCAAGTATTA5060.10891370418521357No Hit
CCTACCTGTGTCGGTTTCAGGTACAGGTGATTCTGGTGTTGGATCTTGCTT5050.10869845971053924No Hit
CCGGTGAGCGGGGGATAGCCCAGAGAAATTTGGATTAATACCCCATACTAT5000.10762223733716755No Hit
GTTAGTGATAGCACAAGACATAACGGAACAGCTTGGAAAGGCTGGCCATAG4990.10740699286249321No Hit
CGTAGTTAGCCGGTGCTTATTCTTACGGTACCGTCATTAGCCCTCTTTATT4950.10654601496379587No Hit
ACCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG4940.10633077048912154No Hit
GCAAGGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTACATTCAG4920.10590028153977288No Hit
GGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAGAG4900.1054697925904242No Hit
GCGTAGGCGGGTATGTAAGTCAGTGGTGAAATACCGGAGCTTAACTTCGGA4890.10525454811574986No Hit
CACAGGTAGACGGGATGAGTATTCTAAGGCGCTCGGGTGAGCCGTGGAGAA4850.10439357021705252No Hit
CACCAAGCCTTCGATCTGTAGCTGGTCTGAGAGGACGACCAGCCACACTGG4750.10224112547030917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)