FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402456
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTGA15370.3819051026696086No Hit
GCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTGCTATCACTAA12310.30587194625002484No Hit
CTCTCTGTCTCCCTCTATCTCTGTCTGTTTCTCTGCCTCTCTGTCTCTCTC8540.2121971097461586No Hit
GGTTGAGCCCGGGGATTTCACAACTGTCTTACATCACCGCCTGCGCACGCT8400.20871846860277896No Hit
GGGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAGA8320.20673067366370484No Hit
CATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAGT8010.19902796827479277No Hit
GCGATAAGCTTCGGGGAGCTGGCAAATTAGCTTTGATCCGGAGATTTCCGA7870.19554932713141313No Hit
CCTAATACGTAGTTAAGTTGAACAAACGAGGTGAGACTTCAATAACAGCCA7440.18486492933388993No Hit
GCAGGTAAGAGGCTTTGGAGAGAGAGAGAGAAAGAGAGAGAGGGAGAGAGA6960.1729381596994454No Hit
GACAGAAAGAGGCAGAGAGACAGGCAGAGGCAGAGAGACAGAGAGAGACAG6530.1622537619019222No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC6490.16125986443238516No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAGT6250.1552964796151629No Hit
GTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATG5850.14535750491979246No Hit
CCCTCTGTCTTGGTCTCTGTCTCTGTCTGCCTTTCTCTGTCTCTCCCCATC5730.14237581251118134No Hit
GATCAGTCGCTGGGTGTAGACCCGAAACCAAGTGATCTATCCATGGCCAGG5550.13790327389826468No Hit
GCGACTGATCGCCCTATTCGGACTCGATTTCTCTACGGCTTCCCTATTCGG5350.13293378655057944No Hit
GCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCCGCT5320.13218836344842666No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC5200.12920667103981553No Hit
GCATTGCTGCAGGGTACGCTTTATCCCGAAGTTACAGCGTCAATTTGCCTA4830.12001311944659789No Hit
CCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGCCGT4670.11603752956844972No Hit
CTTTCTGTCTCTGTTTCTCTGCCTCTTTCTGTCTCTCTGCCTGTCTCTCCA4570.1135527858946071No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG4550.1130558371598386No Hit
CTCTCTGTCTCTGTCTTTCTTTGTCTCTCTGTCTCTGTCTCTCTGCCTCTG4530.11255888842507007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.11081956785338025No Hit
GCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTTC4260.10585008050569503No Hit
CCTACCTGTGTCGGTTTCAGGTACAGGTGATTCTGGTGTTGGATCTTGCTT4190.1041107599340052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.10386228556662094No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGG4120.10237143936231538No Hit
GGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGC4060.10088059315800982No Hit
GAGTACATGGGAGAGGTAGAGAGACGGAGACAGACAGAGAGACAGAGAGAG4060.10088059315800982No Hit
GGCCATAGCGGGTGATAGCCCCGTACGTGAAATGACCTGTGTGGTACTGAG4040.1003836444232413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)