FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007544844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294794
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTGA37241.263255018758862No Hit
GCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTGCTATCACTAA25170.8538165634307346No Hit
GCGATAAGCTTCGGGGAGCTGGCAAATTAGCTTTGATCCGGAGATTTCCGA14980.5081514549142792No Hit
GGTTGAGCCCGGGGATTTCACAACTGTCTTACATCACCGCCTGCGCACGCT13890.47117648256070344No Hit
CATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAGT13770.4671058434025116No Hit
GGCCATAGCGGGTGATAGCCCCGTACGTGAAATGACCTGTGTGGTACTGAG9290.3151353148300169No Hit
GGGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAGA8890.3015665176360442No Hit
GGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGC8540.289693820091318No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGG8130.27578580296749594No Hit
GCGACTGATCGCCCTATTCGGACTCGATTTCTCTACGGCTTCCCTATTCGG7880.267305304721263No Hit
CCATATTCAGACAGGATTTCTCGTGTCCCGCCCTACTTGTCGTTAGCTCAG7850.26628764493171503No Hit
CCCAATCGTGGGGGATAACGCAGCGAAAGCTGTGCTAATACCGCATACGAT7550.25611104703623544No Hit
GTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATG7080.2401677103333175No Hit
ACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTG6820.2313479921572352No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC6220.2109947963662761No Hit
GCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCCCTATTAGTGTGAAGT6150.20862025685733088No Hit
GCGATCAGTAGCTGATGTGAGAGCATGATCAGCCACACGGGCACTGAGACA5960.2021750781901938No Hit
CCTAATACGTAGTTAAGTTGAACAAACGAGGTGAGACTTCAATAACAGCCA5900.2001397586110979No Hit
CCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGCCGT5640.19132004043501563No Hit
GCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCCGCT4960.168253085205262No Hit
GATCAGTCGCTGGGTGTAGACCCGAAACCAAGTGATCTATCCATGGCCAGG4930.167235425415714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.16384322611722085No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC4590.1557019478008372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.1557019478008372No Hit
CGTAGTTAGCCGGTGCTTATTCTTACGGTACCGTCATTAGCCCTCTTTATT4520.15332740829189195No Hit
GCCTACGCACCCTTTAAACCCAGTGAATCCGGATAACGCTTGCACCCTCCG4520.15332740829189195No Hit
GCTATCACCCGCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTG4480.15197052857249468No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCACAGGG4460.15129208871279606No Hit
CTTTATTAGAAAGAGCCGTTTCGTTCCGTACAAAAGCAGTTTACAACCCGA4430.1502744289232481No Hit
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATC4410.14959598906354946No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT4370.1482391093441522No Hit
GGTAGTCCACGCCCTAAACGATGTCAACTGGTTGTTGGGTCTTCACTGACT4320.1465430096949056No Hit
GCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTTC4300.14586456983520696No Hit
CCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGCGTGCTTACAAAAAGTA4230.14349003032626173No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG4030.13670563172927536No Hit
GTTAGTGATAGCACAAGACATAACGGAACAGCTTGGAAAGGCTGGCCATAG4020.13636641179942602No Hit
GCATTGCTGCAGGGTACGCTTTATCCCGAAGTTACAGCGTCAATTTGCCTA3970.13467031215017944No Hit
CTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCT3940.1336526523606315No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC3840.13026045306213832No Hit
GCGCGAATGGAGCGGCCGATGGCAGATTAGGTAGTTGGTGAGGTAAAGGCT3820.12958201320243967No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT3820.12958201320243967No Hit
CACCAAGCCTTCGATCTGTAGCTGGTCTGAGAGGACGACCAGCCACACTGG3800.12890357334274105No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA3650.12381527439500124No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG3650.12381527439500124No Hit
GGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTG3620.1227976146054533No Hit
CCGCATATCTACGCATTTCACTGCTACACGCGGAATTCCATCCCCCTCTGC3580.12144073488605603No Hit
GGCTCAACCTGGGAACTGCATCTGTGACTGCATCGCTGGAGTACGGCAGAG3570.12110151495620672No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC3510.11906619537711079No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG3500.11872697544726148No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCATCATTGACCG3490.11838775551741215No Hit
CCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTAGTTCACTATCGGTCAA3490.11838775551741215No Hit
GCTTATTGATGGGTGTGCGTCTGATTAGGTAGTTGGTGGGGTAGAGGCCCA3410.1156739960786176No Hit
TCTAATAAAGAGGGCTAATGACGGTACCGTAAGAATAAGCACCGGCTAACT3380.11465633628906967No Hit
CCGGTGAGCGGGGGATAGCCCAGAGAAATTTGGATTAATACCCCATACTAT3370.11431711635922033No Hit
GCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCC3350.1136386764995217No Hit
ACTGATCGCAGGCTTGGTGGGCCTCTACCCCACCAACTACCTAATCAGACG3300.1119425768502751No Hit
GATCCAGCCATGCCGCGTGCAGGATGAAGGCCTTCGGGTTGTAAACTGCTT3260.11058569713087783No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCT3120.10583661811298738No Hit
GCGTAGGCGGGTATGTAAGTCAGTGGTGAAATACCGGAGCTTAACTTCGGA3050.10346207860404215No Hit
GGAAATAGCTGGTTCTCTCCGAAAACTATTTAGGTAGTGCCTCAAGTATTA3030.1027836387443435No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA2990.10142675902494623No Hit
CTTGAGTCCGGACGTTAGCACCCGGTGCTCTGTCTCCCAAGCTGTACTCTT2960.10040909923539829No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)