FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007544853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76251990
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG4715600.618423204430468TruSeq Adapter, Index 3 (97% over 37bp)
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAAGATCGGAAGAGCACAC3547920.46528884033059337Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ATCAAGTTCGGAAGAGCACACGTCTGAACTTGATAGATCGGAAGAGCACAC3074730.4032327549746571Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCAAGTCTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG2277440.29867286086566397TruSeq Adapter, Index 3 (97% over 37bp)
CCATAGTTCGGAAGAGCACACGTCTGAACTATGGAGATCGGAAGAGCACAC2026310.26573863842766593Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGAAGGTTCGGAAGAGCACACGTCTGAACCTTCTAGATCGGAAGAGCACAC1832890.24037274305890247Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ATCAAGACTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1581150.20735852270871882TruSeq Adapter, Index 3 (97% over 37bp)
CTATTGTTCGGAAGAGCACACGTCTGAACAATAGAGATCGGAAGAGCACAC1443460.18930128905488236Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CCATAGTCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1420710.18631776036271314TruSeq Adapter, Index 3 (97% over 37bp)
GTGAGGTTCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCGAATCTC1276880.16745530182228685TruSeq Adapter, Index 10 (97% over 35bp)
TGACAGTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCGA1275420.1672638314095147TruSeq Adapter, Index 3 (97% over 39bp)
GAAGTCTGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1205970.1581558723910025TruSeq Adapter, Index 3 (97% over 37bp)
ACAGACTGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1187520.15573626340768287TruSeq Adapter, Index 3 (97% over 37bp)
CCATAGACTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1167930.15316715012945892TruSeq Adapter, Index 3 (97% over 37bp)
ACAGACTTGGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT1115570.14630044409332793No Hit
TCTCTGTTCGGAAGAGCACACGTCTGAACAGAGAAGATCGGAAGAGCACAC1112180.14585586553216512Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ACAGACTTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1087800.14265857192710643No Hit
ACAGACTTAGTGGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTT1067470.13999241200131302No Hit
AGAAGGACCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1000910.1312634594847951TruSeq Adapter, Index 3 (97% over 37bp)
GTGAGGTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG956430.1254301691011605TruSeq Adapter, Index 3 (97% over 37bp)
ACAGACAGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG936630.12283351555808575TruSeq Adapter, Index 3 (97% over 37bp)
TGTAGGTCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG918130.12040734936884925TruSeq Adapter, Index 3 (97% over 37bp)
CTATTGGTTCGGAAGAGCACACGTCTGAACCAATAGAGATCGGAAGAGCAC893810.11721792441089078Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTACCGAGATCGGAAGAGCACAC836930.10975844695987606Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ACAGACTTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTT797560.10459530302094411No Hit
GAAGTCAGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG788280.10337828560277573TruSeq Adapter, Index 3 (97% over 37bp)
ACAGACTAAGTAGTGGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTT781450.10248257127453328No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)