FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007544857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63326339
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGACTTAGTGGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTT4802960.7584458656294658No Hit
CATGACTTGGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTT4331820.6840471229514784No Hit
AGAAGGTCCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC3266420.5158074904661708TruSeq Adapter, Index 13 (97% over 37bp)
CATGACTAGTAGTGGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTT2731610.43135447953181066No Hit
CATGACTTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTT2591490.40922782540768704No Hit
ATCAAGTTCGGAAGAGCACACGTCTGAACTTGATAGATCGGAAGAGCACAC2534040.40015577088705534Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAAGATCGGAAGAGCACAC2478730.3914216484234151Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CATGACTAAGTAGTGGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTT2402900.37944716810488605No Hit
CCATAGTTCGGAAGAGCACACGTCTGAACTATGGAGATCGGAAGAGCACAC2047930.32339308293189034Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CATGACTAGTGGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTT1957830.30916519585949853No Hit
GTGAGGTTCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAAATCTC1815780.2867337712353781TruSeq Adapter, Index 13 (97% over 35bp)
CATGACTTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1776690.28056098426912063No Hit
CATGACTGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTT1721540.27185212775366663No Hit
ATCAAGTCTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC1512880.23890217307525075TruSeq Adapter, Index 13 (97% over 37bp)
CCATAGTCTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC1434520.22652817495102628TruSeq Adapter, Index 13 (97% over 37bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTACCGAGATCGGAAGAGCACAC1431020.22597548233445172Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGAAGGTTCGGAAGAGCACACGTCTGAACCTTCTAGATCGGAAGAGCACAC1354170.21383993159623518Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CATGACTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1259150.1988351166171157No Hit
TCTCTGTTCGGAAGAGCACACGTCTGAACAGAGAAGATCGGAAGAGCACAC1216240.1920591051379111Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ATCAAGACTTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC1205060.19029364700839566TruSeq Adapter, Index 13 (97% over 37bp)
CATGACTTTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1189210.18779073901619356No Hit
GTGAGGTCCTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC1161220.1833707772053584TruSeq Adapter, Index 13 (97% over 37bp)
CCATAGACTATGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC1134920.17921768697224072TruSeq Adapter, Index 13 (97% over 37bp)
AGGATGTCATCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC1115690.1761810358246037TruSeq Adapter, Index 13 (97% over 37bp)
CATGACTGGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTT1089660.17207058187905036No Hit
TGTAGGTCCTACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC1071850.1692581660215665TruSeq Adapter, Index 13 (97% over 37bp)
CATGACTGTAGTGGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTT1068000.16865020414333443No Hit
CATGACTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1062490.16778010805266985No Hit
GAAGTCTGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC1028020.16233687534029087TruSeq Adapter, Index 13 (97% over 37bp)
CATGACTGTGGTATTAATGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTT984870.15552296493880688No Hit
TGTAGGTTCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAAATCTC974460.1538790991849379TruSeq Adapter, Index 13 (97% over 35bp)
CTATTGTTCGGAAGAGCACACGTCTGAACAATAGAGATCGGAAGAGCACAC971610.15342904948287Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CCTTCGTTCGGAAGAGCACACGTCTGAACGAAGGAGATCGGAAGAGCACAC941620.1486932633197065Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CTATTGGTTCGGAAGAGCACACGTCTGAACCAATAGAGATCGGAAGAGCAC915020.14449279943373958Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ACAGACTGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC876710.1384431839648902TruSeq Adapter, Index 13 (97% over 37bp)
AGAAGGACCTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC760940.12016169133036413TruSeq Adapter, Index 13 (97% over 37bp)
GAAGTCAGACTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC754450.11913684130705866TruSeq Adapter, Index 13 (97% over 37bp)
CTCAGCTGCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC749230.11831253974748168TruSeq Adapter, Index 13 (97% over 37bp)
ACAGACAGTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC723330.11422261438482967TruSeq Adapter, Index 13 (97% over 37bp)
CATGACTTGTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT675910.1067344189911247No Hit
CATGACTTAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA672950.10626699894967875No Hit
TGACAGTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAA656530.10367408101706306TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)