FastQCFastQC Report
Fri 16 Dec 2022
EGAF00007544858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63326339
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGACCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC3287720.5191710198184677Illumina Single End PCR Primer 1 (100% over 38bp)
CATGACTCAATAATATCAACACAAAATACTTTTTTTTTTTTTTTTTTTTTT2665680.4209433297573068No Hit
TGTAGGTTCAGACGTGTGCTCTTCCGAACCTACAAGATCGGAAGAGCGTCG2568140.40554057609425365Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATCAAGTTCAGACGTGTGCTCTTCCGAACTTGATAGATCGGAAGAGCGTCG1835490.2898462202275739Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CCATAGTTCAGACGTGTGCTCTTCCGAACTATGGAGATCGGAAGAGCGTCG1812270.2861794994970418Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CATGACTTAATATCAACACAAAATACTTTTTTTTTTTTTTTTTTTTTTTTT1789320.2825554150540741No Hit
ATCAAGACTTGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1531600.24185828901304401Illumina Single End PCR Primer 1 (100% over 38bp)
CATGACTAACAATAATATCAACACAAAATACTTTTTTTTTTTTTTTTTTTT1492450.23567602731621673No Hit
CCATAGACTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1444060.22803465711163248Illumina Single End PCR Primer 1 (100% over 38bp)
AGAAGGTTCAGACGTGTGCTCTTCCGAACCTTCTAGATCGGAAGAGCGTCG1415860.22358153374380288Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CATGACTAAACAATAATATCAACACAAAATACTTTTTTTTTTTTTTTTTTT1404630.22180818000547922No Hit
CACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG1278380.20187176776475269Illumina Single End PCR Primer 1 (100% over 48bp)
GTGAGGACCTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1269360.20044739993575186Illumina Single End PCR Primer 1 (100% over 38bp)
TCTCTGTTCAGACGTGTGCTCTTCCGAACAGAGAAGATCGGAAGAGCGTCG1243170.19631168004201222Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATCAAGTCTTGATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1201130.18967305215607047Illumina Single End PCR Primer 1 (100% over 38bp)
AGGATGACATCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1146480.18104315172869856Illumina Single End PCR Primer 1 (100% over 38bp)
CCATAGTCTATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1130530.17852445251888002Illumina Single End PCR Primer 1 (100% over 38bp)
TGTAGGACCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1112950.1757483564619139Illumina Single End PCR Primer 1 (100% over 38bp)
GAAGTCAGACTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1085360.17139155952154442Illumina Single End PCR Primer 1 (100% over 38bp)
CATGACTAATAATATCAACACAAAATACTTTTTTTTTTTTTTTTTTTTTTT990310.15638200717714No Hit
CTATTGTTCAGACGTGTGCTCTTCCGAACAATAGAGATCGGAAGAGCGTCG966540.15262843474971766Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CCTTCGTTCAGACGTGTGCTCTTCCGAACGAAGGAGATCGGAAGAGCGTCG947930.14968968915130243Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CTATTGGTTCAGACGTGTGCTCTTCCGAACCAATAGAGATCGGAAGAGCGT887730.14018337614621934Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACAGACAGTCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC886490.13998756504777576Illumina Single End PCR Primer 1 (100% over 38bp)
GAAGTCTGACTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC798790.12613866719817798Illumina Single End PCR Primer 1 (100% over 38bp)
AGAAGGTCCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC764300.12069227624227576Illumina Single End PCR Primer 1 (100% over 38bp)
CTCAGCAGCTGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC763770.12060858278890874Illumina Single End PCR Primer 1 (100% over 38bp)
ACAGACTGTCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC730650.1153785315143514Illumina Single End PCR Primer 1 (100% over 38bp)
TTCAAGTTCAGACGTGTGCTCTTCCGAACTTGATAGATCGGAAGAGCGTCG667020.10533057974502522Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TGACTGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG659990.1042204571465911Illumina Single End PCR Primer 1 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)