FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007544965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47037344
Sequences flagged as poor quality0
Sequence length35-51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN873469518.569702830159798No Hit
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAN3270260.6952475888094362Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ATCAAGTTCGGAAGAGCACACGTCTGAACTTGATN3180460.6761563748157209Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTACCGN2610980.5550866137339727Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCTTCGTTCGGAAGAGCACACGTCTGAACGAAGGN2607240.5542915008126309Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CCATAGTTCGGAAGAGCACACGTCTGAACTATGGN2228010.4736683261707974Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGAAGGTTCGGAAGAGCACACGTCTGAACCTTCTN2210370.46991811442414777Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
GTGAGGTTCGGAAGAGCACACGTCTGAACCTCACN2026240.4307726218555197Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CAAGAGTTCGGAAGAGCACACGTCTGAACTCTTGN1794480.3815011323768621Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCTCTGTTCGGAAGAGCACACGTCTGAACAGAGAN1358910.28890024062583125Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGGATGTTCGGAAGAGCACACGTCTGAACATCCTN1285690.27333388551870613Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
TGTAGGTATCGGAAGAGCACACGTCTGAACCTACA1109170.23580625640767472Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
TATCTCGATCGGAAGAGCACACGTCTGAACGAGATA1074970.22853543771519072Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CTATTGTTCGGAAGAGCACACGTCTGAACAATAGN1070260.22753410566719073Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CTATTGGTTCGGAAGAGCACACGTCTGAACCAATAG1041590.2214389485936961Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CGGTAGGTTCGGAAGAGCACACGTCTGAACCTACCG823760.17512893585148007Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
GTGAGGTTCGGAAGAGCACACGTCTGAACCCTCAC821330.17461232504964566Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TGTAGGGATCGGAAGAGCACACGTCTGAACCCTACA733910.15602709200587514Illumina Multiplexing Index Sequencing Primer (96% over 26bp)
TCTCTGGTTCGGAAGAGCACACGTCTGAACCAGAGA658620.14002066103052077Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
GTGAGGTATCGGAAGAGCACACGTCTGAACCTCAC648240.13781390377824054Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ATCAAGGTTCGGAAGAGCACACGTCTGAACCTTGAT636710.13536265993249957Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGGATGGTTCGGAAGAGCACACGTCTGAACCATCCT573780.12198392834425345Illumina Multiplexing Index Sequencing Primer (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)