FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007544968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46304977
Sequences flagged as poor quality0
Sequence length35-51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN802281517.326031713610394No Hit
TGTAGGTTCAGACGTGTGCTCTTCCGAACCTACAN3126480.675193079137044Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATCAAGTTCAGACGTGTGCTCTTCCGAACTTGATN3039710.6564542727232108Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CCTTCGTTCAGACGTGTGCTCTTCCGAACGAAGGN2522870.5448377611762986Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2501640.5402529408447823No Hit
AGAAGGTTCAGACGTGTGCTCTTCCGAACCTTCTN2084630.4501956668718354Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GTGAGGTTCAGACGTGTGCTCTTCCGAACCTCACN1966460.4246757319412987Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCTCTGTTCAGACGTGTGCTCTTCCGAACAGAGAN1287700.27809105703691417Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAAGAGTTCAGACGTGTGCTCTTCCGAACTCTTGN1238190.2673989018502266Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGATGTTCAGACGTGTGCTCTTCCGAACATCCTN1236700.2670771221849435Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TATCTCGTTCAGACGTGTGCTCTTCCGATCGAGATA1099220.23738700917614103Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGTAGGTTCAGACGTGTGCTCTTCCGATACCTACA1076470.23247393039413453Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CTATTGTTCAGACGTGTGCTCTTCCGAACAATAGN1021010.22049681614138367Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTATTGGTTCAGACGTGTGCTCTTCCGAACCAATAG1008250.21774117283332198Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGGTAGGTTCAGACGTGTGCTCTTCCGAACCTACCG838880.18116411114943431Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTGAGGGTTCAGACGTGTGCTCTTCCGAACCTCAC762400.16464752806161637Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AAATAGTTCAGACGTGTGCTCTTCCGAACTATGGN697470.15062527727850938Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCATAGTTCAGACGTGTGCTCTTCCGAACTATGGN656230.14171910721389624Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TCTCTGGTTCAGACGTGTGCTCTTCCGAACCAGAGA640900.138408447973098Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ATCAAGGTTCAGACGTGTGCTCTTCCGAACCTTGAT638100.13780376135377415Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGTAGGGTTCAGACGTGTGCTCTTCCGATCCCTACA626580.13531590783427017Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGAGGTTCAGACGTGTGCTCTTCCGATACCTCAC623170.13457948591573643Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGATGGTTCAGACGTGTGCTCTTCCGAACCATCCT566310.12230002835332365Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ACATAGTTCAGACGTGTGCTCTTCCGAACTATGGN535330.11560960282951872Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)