FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007544969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40775568
Sequences flagged as poor quality0
Sequence length35-51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN659614816.176716410179743No Hit
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAN2636910.64668872301178Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ATCAAGTTCGGAAGAGCACACGTCTGAACTTGATN1969460.48300001608806525Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTACCGN1958390.48028515507129177Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCTTCGTTCGGAAGAGCACACGTCTGAACGAAGGN1957640.4801012213980686Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CAAGAGTTCGGAAGAGCACACGTCTGAACTCTTGN1698440.4165337439321508Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GTGAGGTTCGGAAGAGCACACGTCTGAACCTCACN1683220.4128011165902091Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGAAGGTTCGGAAGAGCACACGTCTGAACCTTCTN1653700.40556148721214624Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CCATAGTTCGGAAGAGCACACGTCTGAACTATGGN1575730.3864397425438684Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTTGAGTTCGGAAGAGCACACGTCTGAACTCAACN1154730.2831916406412781Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGATGTTCGGAAGAGCACACGTCTGAACATCCTN1031580.2529897314980382Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
TATCTCGATCGGAAGAGCACACGTCTGAACGAGATA976040.23936882988362052Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TCTCTGTTCGGAAGAGCACACGTCTGAACAGAGAN833440.20439690748146047Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CTATTGTTCGGAAGAGCACACGTCTGAACAATAGN796960.19545037361588685Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CTATTGGTTCGGAAGAGCACACGTCTGAACCAATAG724370.17764804649686303Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
TGTAGGTATCGGAAGAGCACACGTCTGAACCTACA695730.17062423262871532Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CGGTAGGTTCGGAAGAGCACACGTCTGAACCTACCG570580.13993183369021372Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
GTGAGGTTCGGAAGAGCACACGTCTGAACCCTCAC566360.1388969002222115Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTGAGGTATCGGAAGAGCACACGTCTGAACCTCAC477670.1171461302513309Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ATCAAGGTTCGGAAGAGCACACGTCTGAACCTTGAT428560.10510215332867956Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGGATGGTTCGGAAGAGCACACGTCTGAACCATCCT427900.10494029169624321Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
TGTAGGGATCGGAAGAGCACACGTCTGAACCCTACA417400.10236522027111922Illumina Multiplexing Index Sequencing Primer (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)