FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007544970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40775568
Sequences flagged as poor quality0
Sequence length35-51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN617823515.151806101143706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2793130.6850008809196723No Hit
TGTAGGTTCAGACGTGTGCTCTTCCGAACCTACAN2572340.6308532599717557Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATCAAGTTCAGACGTGTGCTCTTCCGAACTTGATN1922910.4715838661033489Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CCTTCGTTCAGACGTGTGCTCTTCCGAACGAAGGN1919840.4708309642676222Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTGAGGTTCAGACGTGTGCTCTTCCGAACCTCACN1670480.40967669659439204Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGAAGGTTCAGACGTGTGCTCTTCCGAACCTTCTN1599240.39220545008716984Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAAGAGTTCAGACGTGTGCTCTTCCGAACTCTTGN1406710.3449884499463012Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGATGTTCAGACGTGTGCTCTTCCGAACATCCTN1008310.24728288273016824Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TATCTCGTTCAGACGTGTGCTCTTCCGATCGAGATA973600.23877043233340123Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TCTCTGTTCAGACGTGTGCTCTTCCGAACAGAGAN808720.19833445361202573Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTATTGTTCAGACGTGTGCTCTTCCGAACAATAGN769460.18870613893103833Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTATTGGTTCAGACGTGTGCTCTTCCGAACCAATAG710300.17419745078719687Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TGTAGGTTCAGACGTGTGCTCTTCCGATACCTACA675620.1656923577373588Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CCATAGTTCAGACGTGTGCTCTTCCGAACTATGGN589780.14464053572472615Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGGTAGGTTCAGACGTGTGCTCTTCCGAACCTACCG582990.14297532286981263Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTGAGGGTTCAGACGTGTGCTCTTCCGAACCTCAC539880.13240281533294646Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTGAGGTTCAGACGTGTGCTCTTCCGATACCTCAC468210.11482611351974299Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGGTAGTTCAGACGTGTGCTCTTCCGAACTACCGN467520.11465689454037771Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AAATAGTTCAGACGTGTGCTCTTCCGAACTATGGN451120.11063487821923168Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCAAGGTTCAGACGTGTGCTCTTCCGAACCTTGAT429670.10537437516504981Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGGATGGTTCAGACGTGTGCTCTTCCGAACCATCCT425100.10425360598287679Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)