FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007544972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40066390
Sequences flagged as poor quality0
Sequence length35-51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN600967914.999302407828608No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2665300.6652208996118691No Hit
TGTAGGTTCAGACGTGTGCTCTTCCGAACCTACAN2492070.6219851601304734Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATCAAGTTCAGACGTGTGCTCTTCCGAACTTGATN1878420.4688268646114611Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CCTTCGTTCAGACGTGTGCTCTTCCGAACGAAGGN1878130.46875448474394626Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTGAGGTTCAGACGTGTGCTCTTCCGAACCTCACN1643820.4102740476494139Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGAAGGTTCAGACGTGTGCTCTTCCGAACCTTCTN1557000.38860501282995547Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAAGAGTTCAGACGTGTGCTCTTCCGAACTCTTGN1200150.29954033792413043Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGATGTTCAGACGTGTGCTCTTCCGAACATCCTN983060.24535776744548238Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TATCTCGTTCAGACGTGTGCTCTTCCGATCGAGATA910410.22722536270425162Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TCTCTGTTCAGACGTGTGCTCTTCCGAACAGAGAN786740.19635909299540086Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTATTGTTCAGACGTGTGCTCTTCCGAACAATAGN746550.18632824170083703Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTATTGGTTCAGACGTGTGCTCTTCCGAACCAATAG669350.16706022179687263Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TGTAGGTTCAGACGTGTGCTCTTCCGATACCTACA629480.15710923794232523Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGGTAGGTTCAGACGTGTGCTCTTCCGAACCTACCG575430.14361912815204964Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AAATAGTTCAGACGTGTGCTCTTCCGAACTATGGN557480.13913906393862788Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGAGGGTTCAGACGTGTGCTCTTCCGAACCTCAC528640.13194101090714686Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTGAGGTTCAGACGTGTGCTCTTCCGATACCTCAC439890.10979027559008936Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ATCAAGGTTCAGACGTGTGCTCTTCCGAACCTTGAT416510.10395496075388873Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGGATGGTTCAGACGTGTGCTCTTCCGAACCATCCT410890.10255228883860014Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)