FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007544973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42958644
Sequences flagged as poor quality0
Sequence length35-51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN782064118.205046230043948No Hit
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAN3976340.9256204641841116Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ATCAAGTTCGGAAGAGCACACGTCTGAACTTGATN3559710.8286364904814035Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCTTCGTTCGGAAGAGCACACGTCTGAACGAAGGN3100260.7216847906093126Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTACCGN2896140.6741693243390084Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCATAGTTCGGAAGAGCACACGTCTGAACTATGGN2721310.6334720434844265Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGAGGTTCGGAAGAGCACACGTCTGAACCTCACN2469570.5748714973405585Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGAAGGTTCGGAAGAGCACACGTCTGAACCTTCTN2265560.527381637092642Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CAAGAGTTCGGAAGAGCACACGTCTGAACTCTTGN1762110.41018752826555693Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TATCTCGATCGGAAGAGCACACGTCTGAACGAGATA1382360.3217885555233075Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TCTCTGTTCGGAAGAGCACACGTCTGAACAGAGAN1340160.3119651542073814Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
TGTAGGTATCGGAAGAGCACACGTCTGAACCTACA1333390.31038921992044255Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CTATTGTTCGGAAGAGCACACGTCTGAACAATAGN1178310.27428938399452274Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGGATGTTCGGAAGAGCACACGTCTGAACATCCTN1139920.2653528821812905Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CTATTGGTTCGGAAGAGCACACGTCTGAACCAATAG1135880.26441244281360465Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CGGTAGGTTCGGAAGAGCACACGTCTGAACCTACCG852860.1985304750308227Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
TGTAGGGATCGGAAGAGCACACGTCTGAACCCTACA852860.1985304750308227Illumina Multiplexing Index Sequencing Primer (96% over 26bp)
ATCAAGGTTCGGAAGAGCACACGTCTGAACCTTGAT699050.16272627227246744Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
GTTGAGTTCGGAAGAGCACACGTCTGAACTCAACN686480.15980020225964303Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGCTGCGATCGGAAGAGCACACGTCTGAACGCAGCA608830.14172467827429563Illumina Multiplexing Index Sequencing Primer (96% over 26bp)
TCTCTGGTTCGGAAGAGCACACGTCTGAACCAGAGA547880.12753661405141187Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGGATGGTTCGGAAGAGCACACGTCTGAACCATCCT515350.11996421488536743Illumina Multiplexing Index Sequencing Primer (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)