FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007544974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42958644
Sequences flagged as poor quality0
Sequence length35-51
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN730382617.001993824572303No Hit
TGTAGGTTCAGACGTGTGCTCTTCCGAACCTACAN3887130.9048539800278612Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATCAAGTTCAGACGTGTGCTCTTCCGAACTTGATN3493920.8133217612734703Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CCTTCGTTCAGACGTGTGCTCTTCCGAACGAAGGN3054980.7111444206665368Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2612310.608098803118646No Hit
GTGAGGTTCAGACGTGTGCTCTTCCGAACCTCACN2462550.5732373675481935Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGAAGGTTCAGACGTGTGCTCTTCCGAACCTTCTN2195140.5109891271242175Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAAGAGTTCAGACGTGTGCTCTTCCGAACTCTTGN1459520.3397500163180197Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TATCTCGTTCAGACGTGTGCTCTTCCGATCGAGATA1421690.3309438724369419Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGTAGGTTCAGACGTGTGCTCTTCCGATACCTACA1315130.30613862020412Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TCTCTGTTCAGACGTGTGCTCTTCCGAACAGAGAN1310060.3049584153540787Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CCATAGTTCAGACGTGTGCTCTTCCGAACTATGGN1257680.2927652930572017Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CTATTGTTCAGACGTGTGCTCTTCCGAACAATAGN1147900.26721048271449166Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AGGATGTTCAGACGTGTGCTCTTCCGAACATCCTN1124190.2616912209798801Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CTATTGGTTCAGACGTGTGCTCTTCCGAACCAATAG1117680.2601758100185844Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGGTAGGTTCAGACGTGTGCTCTTCCGAACCTACCG874140.20348407645269248Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGTAGGGTTCAGACGTGTGCTCTTCCGATCCCTACA757540.17634169272195835Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCAAGGTTCAGACGTGTGCTCTTCCGAACCTTGAT704800.16406476889726782Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ACATAGTTCAGACGTGTGCTCTTCCGAACTATGGN648850.1510406147829061Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TGCTGCGTTCAGACGTGTGCTCTTCCGATCGCAGCA620180.1443667542206407Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGGTAGTTCAGACGTGTGCTCTTCCGAACTACCGN583840.13590745555190242Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AAATAGTTCAGACGTGTGCTCTTCCGAACTATGGN579940.13499960566725522Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCTCTGGTTCAGACGTGTGCTCTTCCGAACCAGAGA542260.12622837908943307Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGATGGTTCAGACGTGTGCTCTTCCGAACCATCCT525690.12237118098979102Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)