FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007544975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42317203
Sequences flagged as poor quality0
Sequence length35-51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN765060518.07918401412305No Hit
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAN3895630.9205783283928287Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
ATCAAGTTCGGAAGAGCACACGTCTGAACTTGATN3476670.8215736753679111Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCTTCGTTCGGAAGAGCACACGTCTGAACGAAGGN3039800.7183367010338562Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CGGTAGTTCGGAAGAGCACACGTCTGAACTACCGN2846170.6725798961713042Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCATAGTTCGGAAGAGCACACGTCTGAACTATGGN2656960.6278675837814707Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGAGGTTCGGAAGAGCACACGTCTGAACCTCACN2415980.5709214760720362Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGAAGGTTCGGAAGAGCACACGTCTGAACCTTCTN2212860.5229220844298239Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CAAGAGTTCGGAAGAGCACACGTCTGAACTCTTGN1727670.4082665860501224Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TATCTCGATCGGAAGAGCACACGTCTGAACGAGATA1395340.3297335128694588Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGTAGGTATCGGAAGAGCACACGTCTGAACCTACA1308260.30915559329381953Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
TCTCTGTTCGGAAGAGCACACGTCTGAACAGAGAN1304730.30832141717872985Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CTATTGTTCGGAAGAGCACACGTCTGAACAATAGN1160280.27418636340402747Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CTATTGGTTCGGAAGAGCACACGTCTGAACCAATAG1129700.26695998788010633Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGGATGTTCGGAAGAGCACACGTCTGAACATCCTN1120990.26490172330151407Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
CGGTAGGTTCGGAAGAGCACACGTCTGAACCTACCG855480.2021589186790063Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
TGTAGGGATCGGAAGAGCACACGTCTGAACCCTACA853170.20161304139122804Illumina Multiplexing Index Sequencing Primer (96% over 26bp)
ATCAAGGTTCGGAAGAGCACACGTCTGAACCTTGAT697310.16478168464962112Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
GTTGAGTTCGGAAGAGCACACGTCTGAACTCAACN677710.16014999857150294Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGCTGCGATCGGAAGAGCACACGTCTGAACGCAGCA609130.14394382350837318Illumina Multiplexing Index Sequencing Primer (96% over 26bp)
TCTCTGGTTCGGAAGAGCACACGTCTGAACCAGAGA555490.13126812752723757Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGGATGGTTCGGAAGAGCACACGTCTGAACCATCCT513790.121413979085527Illumina Multiplexing Index Sequencing Primer (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)