FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007544980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37671984
Sequences flagged as poor quality0
Sequence length35-51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN622953616.536256757807074No Hit
ATCAAGTTCAGACGTGTGCTCTTCCGAACTTGATN2931620.778196338159413Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TGTAGGTTCAGACGTGTGCTCTTCCGAACCTACAN2897430.7691206282100779Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CCTTCGTTCAGACGTGTGCTCTTCCGAACGAAGGN2331770.6189666039356992Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2327060.6177163379555481No Hit
AGAAGGTTCAGACGTGTGCTCTTCCGAACCTTCTN2119830.5627072893214226Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAAGAGTTCAGACGTGTGCTCTTCCGAACTCTTGN1634540.4338874214854201Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCTCTGTTCAGACGTGTGCTCTTCCGAACAGAGAN1383260.367185333270475Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TATCTCGTTCAGACGTGTGCTCTTCCGATCGAGATA1235120.3278616809775668Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGTAGGTTCAGACGTGTGCTCTTCCGATACCTACA1215410.3226296762071252Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGATGTTCAGACGTGTGCTCTTCCGAACATCCTN1010100.2681302901381568Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CTATTGTTCAGACGTGTGCTCTTCCGAACAATAGN942790.2502629009398602Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTATTGGTTCAGACGTGTGCTCTTCCGAACCAATAG801190.21267528675951872Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGTAGGGTTCAGACGTGTGCTCTTCCGATCCCTACA735260.1951742175299289Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCATAGTTCAGACGTGTGCTCTTCCGAACTATGGN662410.17583623947175175Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AAATAGTTCAGACGTGTGCTCTTCCGAACTATGGN649860.1725048513505421Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGGTAGGTTCAGACGTGTGCTCTTCCGAACCTACCG645340.17130502072840126Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TCTCTGGTTCAGACGTGTGCTCTTCCGAACCAGAGA606080.16088348306794778Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACATAGTTCAGACGTGTGCTCTTCCGAACTATGGN528330.14024480367160913Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ACTCACGTTCAGACGTGTGCTCTTCCGATCGTGAGT522470.13868927105086898Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ATCAAGGTTCAGACGTGTGCTCTTCCGAACCTTGAT518870.1377336537411993Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGCTGCGTTCAGACGTGTGCTCTTCCGATCGCAGCA486620.1291729153420749Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGGATGGTTCAGACGTGTGCTCTTCCGAACCATCCT458620.1217403362668661Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGTAGGTTCAGACGTGTGCTCTTCCGATCTTGTAGGACCTACA419220.11128163571103662Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)