FastQCFastQC Report
Fri 10 May 2024
EGAF00007544981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30583069
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2292320.7495389033716662No Hit
AGAAGGTCGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGG713980.23345596872570246No Hit
TGTAGGTTCGGAAGAGCACACGTCTGAACCTACAN309070.101059184086463Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
AGAAGGTCGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGG306770.10030713398972482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)