FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007544998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007544998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358171
Sequences flagged as poor quality0
Sequence length38-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC23600.6589031496128944No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT18860.5265641271906435No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC11020.30767426731924136No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT10120.28254660483400384No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG6620.18482791739141358No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT5680.1585834699068322No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC4870.13596857367011847No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATGAGCAGTGCT4470.12480072367667958No Hit
ATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC4330.12089197617897597No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT3920.10944492993570111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)