FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5452
Sequences flagged as poor quality0
Sequence length43-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3616.6214233308877475No Hit
CAGGAGCTGGGTGCCCCAGAGGCTGCTGGGTCTTGAGCCACAGCTGCAGCC120.22010271460014674No Hit
GACCGACAGCTCTTTATGGAAGTTTTTGCTGCAAATAGGAATAAGAAATA120.22010271460014674No Hit
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG110.20176082171680115Clontech SMART CDS Primer II A (96% over 26bp)
CTGTGAAGCCAGTGTTCTCTGCTCCCCGGGTCAGAGGTCACAGGTCAAGTT110.20176082171680115No Hit
TCTATAAGGCAGGAATTACTCCATCAGTTTACAGACTCAGAAAGGTGAAAT90.16507703595011006No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTA90.16507703595011006No Hit
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA90.16507703595011006Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGTTTTCTGGGAAAAAGAAACTGCAATGACTTGAAGTTGAGAATGTGG80.1467351430667645No Hit
CATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCAC80.1467351430667645Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT80.1467351430667645Clontech SMART CDS Primer II A (96% over 26bp)
CGCAGAGTGCCGGGATTACAGGTGTGAGCCACTGAGCCCGACCGACAGCTC80.1467351430667645No Hit
GGAATTACAGGAGGCTCGGAAGAGTGCGGAGAAGCGGAAGGCCATGCTGG80.1467351430667645No Hit
GCCAGGTAGCAGGCTTTATTGGGAAGGGACAAAGCCTCAGGAGCTGGGTGC80.1467351430667645No Hit
AGCATCTGTGTGCCATTTGCCACACAGCAACCATCTGGGCTCCCGGCTAC70.12839325018341893No Hit
GTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATG70.12839325018341893Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC70.12839325018341893Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC70.12839325018341893Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTAGGGCTGGGGTGGTTCACCAGATTTGCATGTTCCAAATCACTCTGGCT70.12839325018341893No Hit
CCCCCGACTCAGGTCAAAGTTCATAGCAGCAATGCACAAAGTCCTCTGTG70.12839325018341893No Hit
CCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCATCCAGCCAGAACA70.12839325018341893No Hit
CCTGGTGTCTGGGCTTCCGCAGCTTCTTCTTCTTGAAGTAAGCATCAGTA60.11005135730007337No Hit
ACTTACGTGCCTTCATCCCCAACGAGACTTGCCTACACAGACGACCTGGGT60.11005135730007337No Hit
CACATAAAAGGGCCTATTTCTGCTACCATGTCATATAATTCTCCATTGTG60.11005135730007337No Hit
GCTGGACGGCAGTCTGGAAACGACCAATGAGATCTTGGACTCTGCCTCTC60.11005135730007337No Hit
GCTCACTCCCACAGACTTCCCAGGATGCACCCACCCAGCATCTGTGTGCC60.11005135730007337No Hit
GAAAGGAGGAGGGCGCTCCAGGCGACAGCATTGCAGACGCCATTATCCTCT60.11005135730007337No Hit
GCTTCCAGGAGCTGCTCCAGGACCTAGAGAAGAAGGAGCGCAAGCGCCTC60.11005135730007337No Hit
CCCCACAAAGATAGGTGGCGCGTGTTTCAGGGTCTCTCGTCTCTCTCCTAC60.11005135730007337No Hit
ACAGAGCACCAGGAGAGGTAGCAGTGATTGTAGCTGCTCTGGGTGTATTGT60.11005135730007337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)