FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3423
Sequences flagged as poor quality0
Sequence length41-51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT401.1685655857435RNA PCR Primer, Index 26 (100% over 22bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC391.1393514460999123RNA PCR Primer, Index 26 (96% over 28bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA200.58428279287175RNA PCR Primer, Index 26 (100% over 29bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGC130.3797838153666374TruSeq Adapter, Index 3 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTT120.35056967572305RNA PCR Primer, Index 26 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC110.32135553607946243RNA PCR Primer, Index 26 (100% over 23bp)
GCTTATGGTGACTTTGCGCCACTGCACTCCAGCCTGGGTGACACAGTA100.292141396435875No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC90.26292725679228746No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCT80.23371311714869997No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC70.2044989775051125No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG70.2044989775051125RNA PCR Primer, Index 26 (100% over 25bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT70.2044989775051125RNA PCR Primer, Index 26 (100% over 28bp)
CTTATATTTGGTGCTATGTAAATGAAAATGTTTTTTAGGTTTTCTTGATTT70.2044989775051125No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGA70.2044989775051125No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTC70.2044989775051125No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGT70.2044989775051125No Hit
GCTCTTGAAAAAGGAAAAAGATAGTGTTAAGACAGTGCTTATGGTGACTTT60.175284837861525No Hit
GCTATCTATAAGGGTTGCGTTGACAGCATTATGCTTCTGGCCAAACAG60.175284837861525No Hit
GCACTGGGTGGATATTTCATCATATGCAAGTCTCTGCAACATTCTGCAAGT60.175284837861525No Hit
GCTGGTTAGCCAGGATGTTCTTACAGTAATCCATCCCCTGCCAGCATCC60.175284837861525No Hit
GCTCTGTATGGAGAAGATTAAGCTCATCCACCACTTTGGAAAAATAGTGG60.175284837861525No Hit
CCATGTGAAGGGGCTCCAGCAGGTTGGACATGATCAATACCCATCCAGATT50.1460706982179375No Hit
CCCCTGCCAGCATCCAGTACACGAGGCTTCTCTGTCCCGGCTAGAACCATT50.1460706982179375No Hit
GCTGTTCCGCAGGACCTCCACCTTCGCCCTCACCATCATCGTGGGCGTCAT50.1460706982179375No Hit
GCAAGCAAGGACCCTCTTTGATGAAGTGCAGTTCAAAGAAAAAGTGTTTCT50.1460706982179375No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTA50.1460706982179375No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT50.1460706982179375RNA PCR Primer, Index 26 (96% over 29bp)
CCCTGTCTCTCCCATCTCTCGCTGTCTCTGTCTCATTTTCTCAGTCCGTCT50.1460706982179375No Hit
GCCAGCATCCAGTACACGAGGCTTCTCTGTCCCGGCTAGAACCATTGCCTC50.1460706982179375No Hit
GCACTTTGGGAGGCCGAGGCGGGAGGATCACGAGGTCAGGAGATCGCGACC50.1460706982179375No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTC50.1460706982179375RNA PCR Primer, Index 26 (95% over 23bp)
GCTGTTTGGGGGGGTTGGCGACGGCAGCCCGAGGGCGGCGCAAGGCCTG50.1460706982179375No Hit
TCTCTCCCAGGACCTTCCTAACTTCATGGACATCAGATGCCTTGCGGATG50.1460706982179375No Hit
CCAGAGTGAATTAATAAGATGGTAAAAGAATTCCATTTATCTCCATATAGC40.11685655857434998No Hit
GACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACC40.11685655857434998No Hit
GCTGGAATGAGGCTTTCCAGCTCCATTAAAAACAGGTTTGTCGTGGCCCTC40.11685655857434998No Hit
CCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATG40.11685655857434998No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC40.11685655857434998No Hit
CCATCAATGACCCGGAGCATCCACTGACGCTAGAGGAGTTGAACGTAGT40.11685655857434998No Hit
GATATACAGACAGGCTTGGTTCCACATTCACTACTGCATTTTCAAGCGCC40.11685655857434998No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT40.11685655857434998No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGG40.11685655857434998No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCTT40.11685655857434998RNA PCR Primer, Index 26 (96% over 28bp)
ACTAACTGAGAGCAGAAGGATAAGTATTCTTCACATTCAACAGTGCTTTA40.11685655857434998No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT40.11685655857434998No Hit
CCCTCCTTGAGACTGGAGCTGGCGTCTGCATACGAGAGACTTGGTTGAACT40.11685655857434998No Hit
GCCTCTTTGGAATGCCATTGATTGTTCCATGTACTGTGCATACCCGGA40.11685655857434998No Hit
GCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTATGATGGCAAATAC40.11685655857434998No Hit
TCTCTGTCCCGGCTAGAACCATTGCCTCACTGCTTTATAGATGCTGAGTCT40.11685655857434998No Hit
CCTGTTTCCAGGGTGACGTAGATTTTTTTCCTTCGCCAGTCTCACCGTTAC40.11685655857434998No Hit
AGGAGATCGCGACCATCCTGGCTAACACGGTGAAACCCTGTCTCTACCAA40.11685655857434998No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAGAACCATTTGGATACATAGGT40.11685655857434998No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC40.11685655857434998No Hit
CACTAACTGAGAGCAGAAGGATAAGTATTCTTCACATTCAACAGTGCTTT40.11685655857434998No Hit
AGGAGGGACAGGTGGACTTAATCCTCCTCCTCGTCGTCTCCAGCCCCAGCC40.11685655857434998No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCTTGA40.11685655857434998RNA PCR Primer, Index 26 (96% over 29bp)
ACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGG40.11685655857434998No Hit
CCTGTTCACAAGTTGATATCTTGCATACTGAAACTGTATGATTTTGTTTG40.11685655857434998No Hit
GCAATAACAGCGGCTGATGAAAAACAGGAACTTGCTTACAACCAACAGCTG40.11685655857434998No Hit
CCCTCCACAGTCCATCCCGTGGACCAGCGGCTTCCCACCGGCAAGCTAGCG40.11685655857434998No Hit
CCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGAC40.11685655857434998No Hit
GTCTGGTCCGGCTGTTGAAGGCGCCTTCCAAAGGGCCAGCCCTCGTCGG40.11685655857434998No Hit
GCATTTGGAAGTCGTTGACTGTAGCCACCAGAGTGAATTAATAAGATGGT40.11685655857434998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)