FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3074
Sequences flagged as poor quality0
Sequence length46-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT240.7807417046193884RNA PCR Primer, Index 10 (95% over 21bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC220.715679895901106Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA200.6506180871828237RNA PCR Primer, Index 10 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC190.6180871828236825TruSeq Adapter, Index 10 (96% over 25bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGC180.5855562784645413Illumina PCR Primer Index 5 (95% over 23bp)
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT180.5855562784645413Clontech SMART CDS Primer II A (96% over 26bp)
CTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTGA110.357839947950553Illumina PCR Primer Index 5 (96% over 26bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA110.357839947950553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG100.32530904359141183Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAGAGGCGAGGTTGTTGTGCGAGCGGCGCGTCCTCTCCCGCCCGGGCG80.2602472348731295No Hit
ACAGCAGGCCGGGCGGGCGCGGTGGCTCGCGCCTGTAATCCCAGCACTCTG80.2602472348731295No Hit
CATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCAC70.2277163305139883Clontech SMART CDS Primer II A (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC70.2277163305139883TruSeq Adapter, Index 10 (95% over 22bp)
GCACTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGCTCCAGACC70.2277163305139883No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC70.2277163305139883No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG60.1951854261548471RNA PCR Primer, Index 10 (95% over 24bp)
CCTCTTGTGCAGATTCACAGAGCCAGATCATGGAGTACATTCATAAGAT60.1951854261548471No Hit
TCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC60.1951854261548471Clontech SMART CDS Primer II A (96% over 26bp)
GCTACAGTCGGTCAATCAGTTACTACTGACAGCTACGAAAGAACGAATTG60.1951854261548471No Hit
GGTGTTGGTAGGGCGATGCCTCCCAATAAGGGGAGAATCCTTTCTTCTGCC60.1951854261548471No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTT50.16265452179570591No Hit
CCCGAGTAGCTGGGCCTACAGGCGCCCGCCACCACGCCCGGCTACTTTGTT50.16265452179570591No Hit
CCGAACTGAAGGTTACGAGCGGCCCGTCAGCAGCCCACATGTAACCGAACT50.16265452179570591No Hit
GCAGTAGTTCTGGCTAGAAGGTAGCAAGGAGGGTCGGGTCTCTGCAGTGT50.16265452179570591No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC50.16265452179570591No Hit
CTAGTAGGCAGGGCTTTGTGTAGACCATGACTTTTGGTAGAAGCCAAACG50.16265452179570591No Hit
CAGAAGAAGATGGATTTGATGGTGCAACAGCTGCAGCCCGGAAAGAGGTG50.16265452179570591No Hit
CCTCGAAGCTAGTGATCTTATGTTGTGGTGAAAATCCTTTGATAGCTTCAT50.16265452179570591No Hit
CACAAGAGCCACAGGAAGAAACATTACCAAGGTCCTCATTGCAAACAGAGG50.16265452179570591No Hit
GACATACTGGAAGGCATGGATCACATTGATGAGATACTCATAGTCTTGGT50.16265452179570591No Hit
GCTGGTTGGCCTGGAATTAGGGTGTTTTTTTTTTTTGAGACAGCGTTTCGC50.16265452179570591No Hit
CCCAATAAGGGGAGAATCCTTTCTTCTGCCTTCCGATAGTTGCTGGCAGTC50.16265452179570591No Hit
GCAATAGGGTCCACGCTGGTGACATCCACTTCCTCATCTTCTGCAGCATC50.16265452179570591No Hit
ACTCCGATGGGGCAATTTCACCATTCACCCCCGAATTTCTGGTCCAGCAGC40.13012361743656475No Hit
CCTCTACCGTGTCACCCAGAGCAACCACTCCATGGTTTCTTAGCACCAGG40.13012361743656475No Hit
CTGAGGTGAGAGCCTTGTTGATGGAGTTAAGGTTGGCGTCAGAGGGCATGG40.13012361743656475No Hit
GTCGCCCAGGCTGGAGTGCAGTGGCGCCATCTCGGCTCACTGCAAGCTCCG40.13012361743656475No Hit
AGAGTTGGTTGGCACAGTCGCAGTTGACTGAGCATAGAGGAAACAGGTCTT40.13012361743656475No Hit
CCAGAAAGTACTCCGCTGGGGAGCATGCCAGTTGGGGTCAAGCCTTTCAGC40.13012361743656475No Hit
CCAGCTTCACAGGGCCATGCATGGCATGACCCCCACGGGTGCCATTC40.13012361743656475No Hit
CCTAATTCCAGGCCAACCAGCAGCCCTAAAAAATTAATCTTCGTTTGGCTT40.13012361743656475No Hit
GCTCCGGGGGGTCCCCGGCGGGCCCGGGAGCCGGTGGGCCTTCCGAGAGC40.13012361743656475No Hit
GTTCTGTAGCCCAGGTTGTCCAGCATCCTCATGAGGGCCTCAAACTCATGC40.13012361743656475No Hit
CTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTC40.13012361743656475Clontech SMART CDS Primer II A (96% over 26bp)
CCTGCAAGATCCTGGTGCTAAGAAACCATGGAGTGGTTGCTCTGGGTGAC40.13012361743656475No Hit
CCCACGGGACCGCTCTCGAGGTCCCCCAAGCCAAGGTGAGGCAAGTCCC40.13012361743656475No Hit
GGCACTCCGCAGCCCGTTCAGGACCCCGGCGCGGGCAGGGCGCCCACGAGC40.13012361743656475No Hit
CTGTTGGTTTAGTGGTGCGATCTTGGCTCATCGCAACCTCTGCCTCACGGG40.13012361743656475No Hit
GAGTACATGGGGAGAGCGGAGATGCAGCGGCTTGGGGCCACCCTGCTGTGC40.13012361743656475No Hit
ACAAGCATAGGACCAAGGCAGAAAAGGTCCAGAGGGTTCATGGTCCTCCGT40.13012361743656475No Hit
GATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCC40.13012361743656475No Hit
GGAAGAAGGCAAGGGACGAGACAACCATGCTGTGCTGTATGAGAAGAACC40.13012361743656475No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTC40.13012361743656475No Hit
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC40.13012361743656475Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)