FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2782
Sequences flagged as poor quality0
Sequence length45-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1053.7742631200575123No Hit
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG140.503235082674335Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT140.503235082674335Clontech SMART CDS Primer II A (96% over 26bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC130.46728971962616817Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGGCCGGGCGGGCGCGGTGGCTCGCGCCTGTAATCCCAGCACTCTG100.35945363048166784No Hit
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA90.3235082674335011Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC80.28756290438533433No Hit
CATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCAC70.2516175413371675Clontech SMART CDS Primer II A (96% over 26bp)
GCAAAGCGCTACTGTTGAGGCTATTGAGGCTGATGAAGCTATCAAAGGATT70.2516175413371675No Hit
GGGAAAAGGAAGTGAGTTAAGGACGTACTCGTCTTGGTGAGAGCGTGAGCT60.2156721782890007No Hit
TCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC60.2156721782890007Clontech SMART CDS Primer II A (96% over 26bp)
GCTACAGTCGGTCAATCAGTTACTACTGACAGCTACGAAAGAACGAATTG60.2156721782890007No Hit
GTTCACCACAGGGTCATCCTCAAGATCGTCAATGTTTATCAGCTCGAAGCG50.17972681524083392No Hit
GGAAATAGGGGGCGCGTGTTTGGAAATTGATAGAAAAGATAAAGGGACCG50.17972681524083392No Hit
AATGTTACGAGAAGTAGCAGACAATGAATTGTCAATTAATTCCGCAAGAGC50.17972681524083392No Hit
CTGAGGTGAGAGCCTTGTTGATGGAGTTAAGGTTGGCGTCAGAGGGCATGG50.17972681524083392No Hit
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA50.17972681524083392Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTTCTACCACCACGAGTAACACCGCCCCCACGGGACCGCTCTCGAGGT50.17972681524083392No Hit
CTAGTAGGCAGGGCTTTGTGTAGACCATGACTTTTGGTAGAAGCCAAACG50.17972681524083392No Hit
GCTACTTTGTTCTATTTTTCCTAGAGACGGGCTTTCACCCTGTTAGCCGGG50.17972681524083392No Hit
ACTGAGATGCTGGTAAATGTCCTCAACATCTGCTCAGATGATGAACTAGGG50.17972681524083392No Hit
ACTGGCATGCTCCCCAGCGGAGTACTTTCTGGAGGGAAGCAAACCCTGCA50.17972681524083392No Hit
CCCAAAGTGTTGGGATTACAGGTGTGAGCTGCCACACCCAGCCTAGTCCC40.14378145219266716No Hit
AGTCAAGGGAGGCAGCCGGGAAGCCACAAAAGCCTACAGCAGGCCGGGCGG40.14378145219266716No Hit
CCCAACAAGGGCTTATGGGCCGAGGGGCAGGCTCCAATTTTCAAGCACACG40.14378145219266716No Hit
GGGCTTGCATGGTCCTCACGCCCCACACGCCAAGCCACGTCCGCGCCGCC40.14378145219266716No Hit
CACAAACAGATTAGTGCAGCTGCCACACTGTGAAGAACTAGTCCCACTCTG40.14378145219266716No Hit
GTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATG40.14378145219266716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCGGGCCCGGGAGCCGGTGGGCCTTCCGAGAGCACCGAGGGGGCGGCGCC40.14378145219266716No Hit
TCGAGCAGCGCGCCTATCCGCACGTCTTCACTAAAGGAATCCCCAATGTTC40.14378145219266716No Hit
GGTATCAACGCAGAGTACATGGGAGTAGAGGCGAGGTTGTTGTGCGAGCGG40.14378145219266716No Hit
GCCCGGAAAGAGGTGATAAGGAACAAGATCCGAGCAATAGGCAAAATGGCC40.14378145219266716No Hit
GCACTCTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGCTCCAGACC40.14378145219266716No Hit
GGAATTAGGGTGTTTTTTTTTTTTGAGACAGCGTTTCGCTCCCGTTGCCC40.14378145219266716No Hit
GCCTTCTTCCTGCCTTCTCTCCTTTCCTTCTCTCTCTCCTTTGAGCTTTTT40.14378145219266716No Hit
CCTGTCCTTGGTAAGTGCGCACCCTATGAAGGTTCAGTCTCCGTAATCA40.14378145219266716No Hit
CCTGGTCTGGCAGAAGATTCCTCACAGACAGCAAGACTGCGAGTCAGTTT40.14378145219266716No Hit
GCAATAGGGTCCACGCTGGTGACATCCACTTCCTCATCTTCTGCAGCATC40.14378145219266716No Hit
CCAACAATCAGCAACAACATGGGGACTCCATAGCCGAGAGTCTTGTTCTTG40.14378145219266716No Hit
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC40.14378145219266716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACTCATTTTACACATGCAGGTCAGCAGAAGCCCTAGAGTTGGTTGGCAC30.10783608914450035No Hit
GGAGGGGGGAGGAAAAAATTCAAGAAGCAGAAACCCCTCGCGGAGTTTTAC30.10783608914450035No Hit
CCAGGACATGCGGCTCTAACTTTTATCTGAGTGCTTGTCTGTGAGGGCTG30.10783608914450035No Hit
GCTAAACAAGGGGTGGATTATTCATGAGTTTTCCAGGGGGTGGGCAGTTCC30.10783608914450035No Hit
CCCTGTAGGTGAGGAACCTTGAAGTCTAAGTTTCAGTAGTGTCATAAGTCC30.10783608914450035No Hit
GACCCCAACTGGCATGCTCCCCAGCGGAGTACTTTCTGGAGGGAAGCAAAC30.10783608914450035No Hit
GCCAGTTGTCAGGAAAGCAGAGATTGTGCCTGGTGCTGAATGGCCTTGGGG30.10783608914450035No Hit
CCCTGATTGGACATTTCCTGGAGCTCATTGTCTGAGACCGTCTGGTCCCCC30.10783608914450035No Hit
GCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGG30.10783608914450035No Hit
CCAGAGGGTTCATGGTCCTCCGTTCCACCTGAATCCAGCTAGAGAGCGAGC30.10783608914450035No Hit
CCCGGCTAACAGGGTGAAAGCCCGTCTCTAGGAAAAATAGAACAAAGTAGC30.10783608914450035No Hit
CCTGGGGGTAGGAGGACTATGGTTTGTGGTCCTTTGTGCATAAGAACTTGG30.10783608914450035No Hit
CCCCAAGCCAAGGTGAGGCAAGTCCCAGTTGAATGTCATCCCGTTCCTCTT30.10783608914450035No Hit
GGGCGCGCCGCGCTTCTCCCAGCGCACCGAGGACCGCCCGGGCGCACACA30.10783608914450035No Hit
CCTGAGTAGCTGGGACTACAGGCATGCGCCACCATGCCCAGTTAAGTTTT30.10783608914450035No Hit
TGTTGATGGAGTTAAGGTTGGCGTCAGAGGGCATGGCATCTCTGCGAGGCG30.10783608914450035No Hit
GCTGGCTGTCCACTTTTATGGTCATTTCCTGATTATTTGCTAAACAAGGGG30.10783608914450035No Hit
CCCGGGCGCGCCGCGCTTCTCCCAGCGCACCGAGGACCGCCCGGGCGCAC30.10783608914450035No Hit
CTCAGGAAGTGGCAGCGAAGAAGACTGAGCCCCGACATTCCAGTCTCGACC30.10783608914450035No Hit
GGAGGCAGAGGTTGCAGTGAGCCACGATCACACCATTGCACTCCAGCCTGG30.10783608914450035No Hit
CCCAGACACACAAGTAGAAAAAAACCAATGCTGTGGTTTCTGCCAAGATGG30.10783608914450035No Hit
GTTTGCAAGGAGGGTCTTAGCAGACGTAAGCAAGTTAAAATAAGCTCATAC30.10783608914450035No Hit
GCTATTACTGCCATTGCTGTCCGTGCCGTTAGTCTCTGAGGTGAGAGCCTT30.10783608914450035No Hit
GTGATCCACCCACCTTGGCCTTCCAAAGTGCTGGGATTACAGGTGTGAGCC30.10783608914450035No Hit
GGGAGTAGGAGCCCCTGCCAAGGCAGCAGACAGCAGCTGTCCACCCCAAAT30.10783608914450035No Hit
CCTCCCGGGTTCACGCCATTCCCCGGCCTCAGCCTCCCGAGTAGCTGGGCC30.10783608914450035No Hit
GTCGCCCAGGCTGGAGTGCAGTGGCGCCATCTCGGCTCACTGCAAGCTCCG30.10783608914450035No Hit
GAGTACATGGGAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATC30.10783608914450035No Hit
GCTCCGCGGGCTACGGTCGCTCCCGCCTCTCGAGCGCTGCCGGTGGCCGC30.10783608914450035No Hit
TCTGAAAGATTCCTCCTCTTCATCATCATCAACCATCATTCATTCACTACC30.10783608914450035No Hit
GCTGAAGCAGGAGAATTGCTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCC30.10783608914450035No Hit
GGCTGGATCAGCCCTCACAGACAAGCACTCAGATAAAAGTTAGAGCCGCAT30.10783608914450035No Hit
GCCACCACGCCCGGCTACTTTGTTCTATTTTTCCTAGAGACGGGCTTTCAC30.10783608914450035No Hit
TCCGAGAGCACCGAGGGGGCGGCGCCGGGGTGTCCCCGGCCCGGCGGCCG30.10783608914450035No Hit
CTGTCTGCTCAGGCTCACTCTTATCTTCATCTTCAATATCAAACTCTGATT30.10783608914450035No Hit
GAGTAGAGGCGAGGTTGTTGTGCGAGCGGCGCGTCCTCTCCCGCCCGGGCG30.10783608914450035No Hit
GCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTAC30.10783608914450035Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGTTGGTTTAGTGGTGCGATCTTGGCTCATCGCAACCTCTGCCTCACGGG30.10783608914450035No Hit
CCTCTATAATTTTCTTGTGTTTTATGGTTCAGGTCTGTGCTTGAAGTAGTG30.10783608914450035No Hit
GCTGGGCTCGGTATTCCCCTTCCTGCTAGTCCTGGTGGATCTGCAGTACG30.10783608914450035No Hit
CCATCCTGGCCGGGGAGGTCAGGGCCTGGGCGGCCAGGGCCCATTTCG30.10783608914450035No Hit
CCATTATCTCCACAAAATCCTGTGACAGCAACTTATCTGCCTCATCCAAT30.10783608914450035No Hit
GGCTGGAGTGCAGTGGCGCGATCTCGACTCACTGCAAGCTCCGCCTCCCGG30.10783608914450035No Hit
GGCATGGAAGGCCCTGAAAAATCTGTTTCCTGTGGAGGGAGAAAGGCTTGG30.10783608914450035No Hit
GCTGGCCCCAGGCTGTTAAGACTAAGGGGTCGAGGGAAAAGAGGCAGAGCC30.10783608914450035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)