FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677
Sequences flagged as poor quality0
Sequence length46-51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT792.1484906173511016Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG641.7405493608920315Clontech SMART CDS Primer II A (96% over 26bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC611.6589611096002175Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA521.4141963557247756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC350.9518629317378298Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCAC320.8702746804460157Clontech SMART CDS Primer II A (96% over 26bp)
TCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC210.5711177590426979Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA190.5167255915148219Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGAT190.5167255915148219Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT140.3807451726951319Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT140.3807451726951319Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT140.3807451726951319Clontech Universal Primer Mix Long (100% over 23bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG120.3263530051672559Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTAC120.3263530051672559Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCGAGACACCACTCACACCCATCCCGAGACACCACTCACACCCATCCCG120.3263530051672559No Hit
GTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATG110.2991569214033179Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCT110.2991569214033179Clontech SMART CDS Primer II A (96% over 26bp)
GTCTCGGGATGGGTGTGAGTGGTGTCTCGGGATGGGTGTGAGTGGTGTCTC90.24476475387544194No Hit
ATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGC90.24476475387544194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAAGAAATAGGTAACATCAAAACGTTTACGACAAAGGTACACCACAAATG80.21756867011150394No Hit
CCACTGGGCTTCCCACAAAGGCAATGATGCGCATCTTGTGATTCTTGCCTT70.19037258634756596No Hit
GTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTAC70.19037258634756596Clontech SMART CDS Primer II A (96% over 26bp)
ACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCG70.19037258634756596Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTC60.16317650258362795Clontech SMART CDS Primer II A (96% over 26bp)
TCTCCGGGAGCATTATCCCCACAGGCTGGCTCTCTGGCTCTCTAGCTCC60.16317650258362795No Hit
AGGGAAACTTTAGGCTAGATACAAAATTCACACAGGGAAAGGCACGGACTC60.16317650258362795No Hit
CCCAGTGCTGCTGGGCTCAATGGGAAGTCCAAAGTCAGAGGTAGCACCAGC50.13598041881968997No Hit
CTCGTCGCCCCGCTTCACGTGCAGCAGAACCATGGCGGTCCCGCCGAGGT50.13598041881968997No Hit
GCTCTGTTGCCTAGGCTGGAGTGCAGTGGCGCAATCTCGGCTCACTGCAAC50.13598041881968997No Hit
GAGTACATGGGGAGGAATTGTGTCCTATGTGACTCTGCTGGGAGAGGACTG50.13598041881968997No Hit
GCATATTAGGAGGGAGAAATATGCCATGTTCGGCTAATTCTTCCATTTCTG50.13598041881968997No Hit
CCTTGAGCCGCCCATTATAGACCCGGGCCACCTGCACCGTGAGCTCCTCC50.13598041881968997No Hit
TGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGAT50.13598041881968997Clontech SMART CDS Primer II A (96% over 26bp)
CTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATAC50.13598041881968997Clontech SMART CDS Primer II A (96% over 26bp)
GCTGAGTGGCTTGTCATCATTCTCGAAAATTCTGAGACTAGTGAGCTCTCT50.13598041881968997No Hit
GGGAAGTGGATGGCGTGGAGATATGGCGCAACTGCGGCGCGGACACTTGAC50.13598041881968997No Hit
GGGTGTGAGTGGTGTCTCGGGATGGGTGTGCGTGGTGTCACGGGATGGGTG50.13598041881968997No Hit
CCGGCTGCGTGGCCCGCGGGTCCTGCCGGCCGAGGGTCGCCGGATCGCC50.13598041881968997No Hit
CCATCATTCCAATGAGGCTTGAGATGTTTTTTTAACACTTCCATCACACCG40.10878433505575197No Hit
GGTTTCCCTGGACTTTGTCCATGCGCTTTTTTCCTTTGCTGATTTTGCTTC40.10878433505575197No Hit
GCACTGAGTTGGGTATTGAAGAGTGGAATGGGTTTGAATGTGAAGGAATGG40.10878433505575197No Hit
CAGCCTCCCGAGTAGCTGGGACTACAGGCGCTCGCCACCACGCCTGGCTA40.10878433505575197No Hit
GACGGAATTACACTTTCTGGCAATGGTTTGACGGTGTGATGGAAGTGTTA40.10878433505575197No Hit
GGAAGGAAGGAAGGAAAGAGAGAGAGGAAGAAAGAGAGAGGAAGGAAGGA40.10878433505575197No Hit
CCCATTATAGACCCGGGCCACCTGCACCGTGAGCTCCTCCAGCTCGGTACT40.10878433505575197No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC40.10878433505575197Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGGGGAGACTGGGAAGGTCCTCAGCCATTCAGCACCATGCGGACG40.10878433505575197No Hit
TCAACAAGGAGAGGGAAGTAAAAAAGGGAAAGGAAAGAAAAAGAAGTAACC40.10878433505575197No Hit
GATAGAGAACAGCATACTTCCTTTCAAGATCATGAACTTCCTCATAGAATT40.10878433505575197No Hit
CCGATACAAATTAGAAGTCTCCATTCATGTCTCTTACAGTAGTACCACAT40.10878433505575197No Hit
CCTTGCTGAGGATGTAAACCTGGGCCTCCACCTTCTGGTGGGGCTTGATGG40.10878433505575197No Hit
GATATAGTTTGGCTGTGTCCCCACCCAAATCTCATCTTGAACTGTAGTTCC40.10878433505575197No Hit
GCTTCTTAAAGTTCATTCCATCCAGGTAGAGCTGCTCCATGTTTCTAACTG40.10878433505575197No Hit
GTCTGGTGCCGGCGTTTGGGCTACGGTTGTTGTTGGCGACTGTGCTTCA40.10878433505575197No Hit
TCCCGAGACACCACTCACACCCATCCCGAGACACCACTGCACACCCATCCC40.10878433505575197No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC40.10878433505575197Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTCAGGGAGAACATGACAGGCATGAAGTGGGACACAAAGGGCTTGT40.10878433505575197No Hit
GCTAGAGTGCAGTGGTGCAAACATGGCGCACTGCAGCCTTGAATTCCTGGG40.10878433505575197No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC40.10878433505575197Clontech Universal Primer Mix Long (96% over 26bp)
GACAGTTCCTTCGTGTTGAATCTGCTGCAACATACTGTCTAGCACAATAT40.10878433505575197No Hit
CCCACCCCACCTCTTAAGAACTGCTGAAGATAGAGGCTAGAAGCTTCTGTG40.10878433505575197No Hit
GCCACCAGCATCTCTACTTCTTCCTCTGTCATCTTCTCACCCAGTGTGAC40.10878433505575197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)