FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007545443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007545443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677
Sequences flagged as poor quality0
Sequence length46-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1193.236333967908621No Hit
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC782.121294533587163Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT701.9037258634756595Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG601.6317650258362797Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA451.2238237693772096Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC411.1150394343214578Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATG330.8974707642099536Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCAC310.8430785966820777Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGA260.7070981778623878Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT200.5439216752787599Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG170.46233342398694594Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT130.3535490889311939Clontech Universal Primer Mix Long (100% over 23bp)
TCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACC120.3263530051672559Clontech SMART CDS Primer II A (96% over 26bp)
GCTCACCGCGGGCTAGGGAGCGTGGGATTCCGGACTGTGAGCGGCTGTT90.24476475387544194No Hit
GATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCT90.24476475387544194Clontech SMART CDS Primer II A (96% over 26bp)
TCCCGAGACACCACTCACACCCATCCCGAGACACCACTCACACCCATCCCG90.24476475387544194No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT80.21756867011150394Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGC80.21756867011150394Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAAGAAATAGGTAACATCAAAACGTTTACGACAAAGGTACACCACAAATG70.19037258634756596No Hit
GCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGAT70.19037258634756596Clontech SMART CDS Primer II A (96% over 26bp)
CCGCCTCCCGGGTTCAAGCGATTCTCCTGCCTCGGGATGGGTGTGAGTGGT70.19037258634756596No Hit
GTCCCTGCCAGTGGTGGTGATCGTTCATGGCAGCCAGGACAACAATGCGAC60.16317650258362795No Hit
CTCAAGTCTAGCCTTTGAGAACTGCTGCAGAATCCTTCTCCGGGAGCATT60.16317650258362795No Hit
CTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTC60.16317650258362795Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTAC60.16317650258362795Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTCGGGATGGGTGTGAGTGGTGTCTCGGGATGGGTGTGAGTGGTGTCTC50.13598041881968997No Hit
TATCAAATAGAGGCTGATAGAGAACAGCATACTTCCTTTCAAGATCATGA50.13598041881968997No Hit
TCCCGAGACACCACTGCACACCCATCCCGAGACACCACTCACACCCATCCC50.13598041881968997No Hit
CCAGGCTGCAGGCCCAGCAGGATGCTGTCAACATAGTTTGTCATTCAA50.13598041881968997No Hit
GATTGAGAAAGGGAACATGACAAACACCCTGCAAGTCTCCCTTGTGCCCTG50.13598041881968997No Hit
TCCCGAGACACCACTCACACCCATCCCGAGACACCACGCACACCCATCCCG50.13598041881968997No Hit
CCCGAGACACCACTCACACCCATCCCGAGACACCACTCACACCCATCCCG50.13598041881968997No Hit
GCTGAAATCCGGCATGTTCTTGTCACACTGGGTGAGAAGATGACAGAGGA50.13598041881968997No Hit
CTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATAC50.13598041881968997Clontech SMART CDS Primer II A (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA50.13598041881968997Clontech Universal Primer Mix Long (96% over 26bp)
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG50.13598041881968997No Hit
CTCTCCCACTCGCCTTATTTTCCTGTTCGCCACTGCGGGGACTTCTAATTT50.13598041881968997No Hit
GTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAAC40.10878433505575197Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTGCTGACAGAAACTTACAAACTGGGTCAAAAACAACATTCATTTAGA40.10878433505575197No Hit
GCAAGATGGCGGCGGCTGGGGCTGGCCGTCTGAGGCGGGTGGCATCGGCTC40.10878433505575197No Hit
GGTTTCCCTGGACTTTGTCCATGCGCTTTTTTCCTTTGCTGATTTTGCTTC40.10878433505575197No Hit
GCCTCGGGATGGGTGTGAGTGGTGTCTCGGGATGGGTGTGCGTGGTGTCAC40.10878433505575197No Hit
CCACATTATGTTCCCATATCATTCTCCAGAAATCCTTGATGGTATGAAGG40.10878433505575197No Hit
CCGGTTGCTCTGCACTTCGGCCTTGAATTTCATGTTGAGCGCCTCACAC40.10878433505575197No Hit
GGGAAACTTTAGGCTAGATACAAAATTCACACAGGGAAAGGCACGGACTCT40.10878433505575197No Hit
CCACCTCCTAGTCTGATGATGTACTAGGAGATTCACAGACTCAGCCTGT40.10878433505575197No Hit
CCCAGAAAGGAAGACCCAGTGGACGTGTGGTCACACAGTATCACTACACGC40.10878433505575197No Hit
GGCAGGGTGCCATTTGCCGTGCCTGACAAAGTGCTGTGGCCACAGCTGTGT40.10878433505575197No Hit
GTAGGTCATGGGCCTGTTGCTTGTTTACAAACCCCAAAATGGCCCCATCAT40.10878433505575197No Hit
CCCAAAGTGCTGGGATTACAGACGTGAGCCACCGCACCCAGCCAGGACTAC40.10878433505575197No Hit
GCCAGTGGTGGTGATCGTTCATGGCAGCCAGGACAACAATGCGACGGCCAC40.10878433505575197No Hit
GATCAAGAGTCCCACTGATGGCCAGGCGCGGTGGCTCACGCCTGTAATCCC40.10878433505575197No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT40.10878433505575197No Hit
AGGGAAACTTTAGGCTAGATACAAAATTCACACAGGGAAAGGCACGGACTC40.10878433505575197No Hit
GAGTTCAAGGAGGCCTTCCAGCTGTTTGACCGAACAGGTGATGGCA40.10878433505575197No Hit
GCTGAGTGGCTTGTCATCATTCTCGAAAATTCTGAGACTAGTGAGCTCTCT40.10878433505575197No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC40.10878433505575197Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCATCTTCTCATTTGGAGCTTGCCCATTCCTTCGTCCAATATCATCCT40.10878433505575197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)